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. 2019 Dec 6;11(12):2027.
doi: 10.3390/polym11122027.

Trehalose-Rich, Degradable Hydrogels Designed for Trehalose Release under Physiologically Relevant Conditions

Małgorzata Burek  1   2 , Ilona Wandzik  1   2
Affiliations

Trehalose-Rich, Degradable Hydrogels Designed for Trehalose Release under Physiologically Relevant Conditions

Małgorzata Burek et al. Polymers (Basel). .

Abstract

Trehalose, a natural disaccharide, is primarily known for its ability to protect proteins from inactivation and denaturation caused by a variety of stress conditions. Furthermore, over the past few years, it has emerged as a promising therapeutic candidate for treatment of neurodegenerative diseases. Herein, we examine the attachment of trehalose to polymers for release under selected physiologically relevant conditions. The proposed strategies are evaluated specifically using hydrogels undergoing simultaneous degradation during trehalose release. These materials are fabricated via copolymerization of the appropriate acrylamide-type monomers with polymerizable trehalose esters or benzylidene acetals. This provides trehalose release in a slightly alkaline (i.e., pH 7.4) or mildly acidic (i.e., pH 5.0) environment, respectively. Using this method materials containing up to 51.7 wt% of trehalose are obtained. The presented results provide a solid basis for future studies on polymeric materials intended for trehalose release in biological systems.

Keywords: glycopolymer; hydrogel; hydrolytic degradation; trehalose; trehalose delivery.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Structures of trehalose monomers and schematic presentation of polymer network of trehalose-rich, degradable hydrogels.
Figure 2
Figure 2
1H NMR spectra of trehalose (A) and degradation products of selected hydrogels (B–D) with magnification of the section of sugar proton signals (E).
Figure 3
Figure 3
Trehalose release profiles from N,N-dimethylacrylamide (DMAM)-based hydrogels containing benzylidene acetals of trehalose at pH 5.0, 37 °C. Thunder signs show visual degradation of hydrogels into aqueous soluble products.
Figure 4
Figure 4
Trehalose release profiles from acrylamide (AM)-based hydrogels containing trehalose acrylates at pH 7.4, 37 °C. Thunder signs show visual degradation of hydrogels into aqueous soluble products.
See this image and copyright information in PMC

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