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. 2019 Dec;5(12):1260-1272.
doi: 10.1038/s41477-019-0562-1. Epub 2019 Dec 2.

Prevalent cytidylation and uridylation of precursor miRNAs in Arabidopsis

Jianbo Song #  1   2 , Xiaoyan Wang #  1 , Bo Song  1 , Lei Gao  1 , Xiaowei Mo  1 , Luming Yue  1 , Haiqi Yang  1 , Jiayun Lu  1 , Guodong Ren  3 , Beixin Mo  4 , Xuemei Chen  5
Affiliations

Prevalent cytidylation and uridylation of precursor miRNAs in Arabidopsis

Jianbo Song et al. Nat Plants. 2019 Dec.

Abstract

A key step in microRNA biogenesis is the processing of a primary precursor RNA by the microprocessor into a precursor miRNA (pre-miRNA) intermediate. In plants, little is known about the processes that act on pre-miRNAs to influence miRNA biogenesis. Here, we performed 3' rapid amplification of complementary DNA ends sequencing to profile pre-miRNA 3' ends in Arabidopsis. 3' end heterogeneity was prevalent, and the three microprocessor components promoted 3' end precision. Extensive cytidylation and uridylation of precise and imprecise pre-miRNA 3' ends were uncovered. The nucleotidyl transferase HESO1 uridylated pre-miRNAs in vitro and was responsible for most pre-miRNA uridylation in vivo. HESO1, NTP6 and NTP7 contribute to pre-miRNA cytidylation. Tailing of pre-miRNAs tended to restore trimmed pre-miRNAs to their intact length to promote further processing. In addition, HESO1-mediated uridylation led to the degradation of certain imprecisely processed pre-miRNAs. Thus, we uncovered widespread cytidylation and uridylation of pre-miRNAs and demonstrated diverse functions of pre-miRNA tailing in plants.

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