In vitro culture of cynomolgus monkey embryos beyond early gastrulation

doi:10.1126/science.aax7890

. 2019 Nov 15;366(6467):eaax7890.

doi: 10.1126/science.aax7890. Epub 2019 Oct 31.

In vitro culture of cynomolgus monkey embryos beyond early gastrulation

Huaixiao Ma^#^{1

2

3}, Jinglei Zhai^#^{1

3

4}, Haifeng Wan^#^{1

3}, Xiangxiang Jiang^#^{1

3}, Xiaoxiao Wang^{1

3

4}, Lin Wang², Yunlong Xiang¹, Xiechao He⁵, Zhen-Ao Zhao¹, Bo Zhao², Ping Zheng^{6

5

7}, Lei Li^{8

3

4}, Hongmei Wang^{8

3

4}

Affiliations

¹ State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
² State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
³ Innovation Academy for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China.
⁴ Institute of Zoology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China.
⁵ Primate Research Center, Yunnan Key Laboratory of Animal Reproduction, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
⁶ State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China. lil@ioz.ac.cn zhengp@mail.kiz.ac.cn wanghm@ioz.ac.cn.
⁷ Center for Excellence in Animal Evolution and Genetics, Chinese Academy of Sciences, Kunming 650223, China.
⁸ State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. lil@ioz.ac.cn zhengp@mail.kiz.ac.cn wanghm@ioz.ac.cn.

^# Contributed equally.

PMID: 31672918
DOI: 10.1126/science.aax7890

In vitro culture of cynomolgus monkey embryos beyond early gastrulation

Huaixiao Ma et al. Science. 2019.

. 2019 Nov 15;366(6467):eaax7890.

doi: 10.1126/science.aax7890. Epub 2019 Oct 31.

Authors

Affiliations

¹ State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
² State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
³ Innovation Academy for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China.
⁴ Institute of Zoology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China.
⁵ Primate Research Center, Yunnan Key Laboratory of Animal Reproduction, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
⁶ State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China. lil@ioz.ac.cn zhengp@mail.kiz.ac.cn wanghm@ioz.ac.cn.
⁷ Center for Excellence in Animal Evolution and Genetics, Chinese Academy of Sciences, Kunming 650223, China.
⁸ State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. lil@ioz.ac.cn zhengp@mail.kiz.ac.cn wanghm@ioz.ac.cn.

^# Contributed equally.

PMID: 31672918
DOI: 10.1126/science.aax7890

Abstract

Gastrulation is a key event in embryonic development when the germ layers are specified and the basic animal body plan is established. The complexities of primate gastrulation remain a mystery because of the difficulties in accessing primate embryos at this stage. Here, we report the establishment of an in vitro culture (IVC) system that supports the continuous development of cynomolgus monkey blastocysts beyond early gastrulation up to 20 days after fertilization. The IVC embryos highly recapitulated the key events of in vivo early postimplantation development, including segregation of the epiblast and hypoblast, formation of the amniotic and yolk sac cavities, appearance of the primordial germ cells, and establishment of the anterior-posterior axis. Single-cell RNA-sequencing analyses of the IVC embryos provide information about lineage specification during primate early postimplantation development. This system provides a platform with which to explore the characteristics and mechanisms of early postimplantation embryogenesis in primates with possible conservation of cell movements and lineages in human embryogenesis.

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Primate embryos grown in the lab for longer than ever before.
Cyranoski D. Cyranoski D. Nature. 2019 Nov;575(7781):17-18. doi: 10.1038/d41586-019-03326-5. Nature. 2019. PMID: 31690853 No abstract available.
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Neupane J, Wong FCK, Surani MA. Neupane J, et al. Cell Res. 2020 Feb;30(2):103-104. doi: 10.1038/s41422-019-0269-x. Cell Res. 2020. PMID: 31913357 Free PMC article. No abstract available.
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In vitro culture of cynomolgus monkey embryos beyond early gastrulation

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In vitro culture of cynomolgus monkey embryos beyond early gastrulation

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