Synchronized Rayleigh and Raman scattering for the characterization of single optically trapped extracellular vesicles

doi:10.1016/j.nano.2019.102109

. 2020 Feb:24:102109.

doi: 10.1016/j.nano.2019.102109. Epub 2019 Oct 24.

Synchronized Rayleigh and Raman scattering for the characterization of single optically trapped extracellular vesicles

Agustin Enciso-Martinez¹, Edwin van der Pol², Aufried T M Lenferink³, Leon W M M Terstappen⁴, Ton G van Leeuwen⁵, Cees Otto⁶

Affiliations

¹ Department of Medical Cell BioPhysics, TechMed Centre, University of Twente, Enschede, The Netherlands. Electronic address: a.encisomartinez@utwente.nl.
² Department of Biomedical Engineering and Physics, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, The Netherlands; Laboratory Experimental Clinical Chemistry, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, The Netherlands; Vesicle Observation Center, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, The Netherlands. Electronic address: e.vanderpol@amsterdamumc.nl.
³ Department of Medical Cell BioPhysics, TechMed Centre, University of Twente, Enschede, The Netherlands.
⁴ Department of Medical Cell BioPhysics, TechMed Centre, University of Twente, Enschede, The Netherlands. Electronic address: l.w.m.m.terstappen@utwente.nl.
⁵ Department of Biomedical Engineering and Physics, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, The Netherlands; Vesicle Observation Center, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, The Netherlands. Electronic address: t.g.vanleeuwen@amsterdamumc.nl.
⁶ Department of Medical Cell BioPhysics, TechMed Centre, University of Twente, Enschede, The Netherlands. Electronic address: c.otto@utwente.nl.

PMID: 31669420
DOI: 10.1016/j.nano.2019.102109

Free article

Synchronized Rayleigh and Raman scattering for the characterization of single optically trapped extracellular vesicles

Agustin Enciso-Martinez et al. Nanomedicine. 2020 Feb.

Free article

. 2020 Feb:24:102109.

doi: 10.1016/j.nano.2019.102109. Epub 2019 Oct 24.

Authors

Agustin Enciso-Martinez¹, Edwin van der Pol², Aufried T M Lenferink³, Leon W M M Terstappen⁴, Ton G van Leeuwen⁵, Cees Otto⁶

Affiliations

¹ Department of Medical Cell BioPhysics, TechMed Centre, University of Twente, Enschede, The Netherlands. Electronic address: a.encisomartinez@utwente.nl.
² Department of Biomedical Engineering and Physics, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, The Netherlands; Laboratory Experimental Clinical Chemistry, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, The Netherlands; Vesicle Observation Center, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, The Netherlands. Electronic address: e.vanderpol@amsterdamumc.nl.
³ Department of Medical Cell BioPhysics, TechMed Centre, University of Twente, Enschede, The Netherlands.
⁴ Department of Medical Cell BioPhysics, TechMed Centre, University of Twente, Enschede, The Netherlands. Electronic address: l.w.m.m.terstappen@utwente.nl.
⁵ Department of Biomedical Engineering and Physics, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, The Netherlands; Vesicle Observation Center, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, The Netherlands. Electronic address: t.g.vanleeuwen@amsterdamumc.nl.
⁶ Department of Medical Cell BioPhysics, TechMed Centre, University of Twente, Enschede, The Netherlands. Electronic address: c.otto@utwente.nl.

PMID: 31669420
DOI: 10.1016/j.nano.2019.102109

Abstract

Extracellular Vesicles (EVs) can be used as biomarkers in diseases like cancer, as their lineage of origin and molecular composition depend on the presence of cancer cells. Recognition of tumor-derived EVs (tdEVs) from other particles and EVs in body fluids requires characterization of single EVs to exploit their biomarker potential. We present here a new method based on synchronized Rayleigh and Raman light scattering from a single laser beam, which optically traps single EVs. Rapidly measured sequences of the Rayleigh scattering amplitude show precisely when an individual EV is trapped and the synchronously acquired Raman spectrum labels every time interval with chemical information. Raman spectra of many single EVs can thus be acquired with great fidelity in an automated manner by blocking the laser beam at regular time intervals. This new method enables single EV characterization from fluids at the single particle level.

Keywords: Exosomes; Extracellular vesicles; Nanomedicine; Nanoparticles; Raman spectroscopy; Single particle analysis.

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Synchronized Rayleigh and Raman scattering for the characterization of single optically trapped extracellular vesicles

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Synchronized Rayleigh and Raman scattering for the characterization of single optically trapped extracellular vesicles

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