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Review
. 2019 Oct 4;20(19):4926.
doi: 10.3390/ijms20194926.

Scaffold-Free 3-D Cell Sheet Technique Bridges the Gap between 2-D Cell Culture and Animal Models

Ayidah Alghuwainem  1 , Alaa T Alshareeda  2 , Batla Alsowayan  3
Affiliations
Review

Scaffold-Free 3-D Cell Sheet Technique Bridges the Gap between 2-D Cell Culture and Animal Models

Ayidah Alghuwainem et al. Int J Mol Sci. .

Abstract

Various tissue engineering techniques have been created in research spanning two centuries, resulting in new opportunities for growing cells in culture and the creation of 3-D tissue-like constructs. These techniques are classified as scaffold-based and scaffold-free techniques. Cell sheet, as a scaffold-free technique, has attracted research interest in the context of drug discovery and tissue repair, because it provides more predictive data for in vivo testing. It is one of the most promising techniques and has the potential to treat degenerative tissues such as heart, kidneys, and liver. In this paper, we argue the advantages of cell sheets as a scaffold-free approach, compared to other techniques, including scaffold-based and scaffold-free techniques such as the classic systemic injection of cell suspension.

Keywords: 3-dimensional (3-D) cell culture; cell sheet technique; scaffold-based technique; solid tumor model.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
An illustration of standard cell detachment vs cell sheet detachment. (A) Cells harvested through enzymatic digestion. In this approach, cells lose their cell–cell junctions which cause them to float in a regular culture dish. (B) Cell sheet detachment through temperature control without any enzymatic digestion using temperature responsive culture dish. The temperature is lowered from 37 to 20 °C, which maintains the cell–cell junctions and ECM surface.
See this image and copyright information in PMC

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