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. 2019 Sep 23;14(1):107.
doi: 10.1186/s13000-019-0882-5.

Increased expression of miR-601 is associated with poor prognosis and tumor progression of gastric cancer

Cuili Min  1 , Aixia Zhang  2 , Jing Qin  2
Affiliations

Increased expression of miR-601 is associated with poor prognosis and tumor progression of gastric cancer

Cuili Min et al. Diagn Pathol. .

Abstract

Background: MicroRNAs (miRNAs) have been considered to participate in many tumorigenesis, including gastric cancer (GC). Abnormal expression of miR-601 has been reported in GC, but its role is not clear. The goal of this study is to explore the expression patterns, clinical value and functional role of miR-601 in GC.

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to evaluate the expression level of miR-601. The association between miR-601 expression and overall survival was estimated by the Kaplan-Meier survival method. The significance of different variables with respect to survival was analyzed by using the Cox regression assay. Cell experiments were applied to investigate the functional role of miR-601 in GC.

Results: We found that miR-601 was significantly up-regulated in GC tissues and cells compared with the controls (all P < 0.01). The levels of miR-601 expression were significantly associated with TNM stage, lymph node metastasis, lymphatic invasion, and distant metastasis (all P < 0.05). Kaplan-Meier survival analysis showed that patients in the high miR-601 expression group had poor overall survival (log-rank P = 0.001). Moreover, we confirmed that miR-601, TNM stage, and distant metastasis were independent prognostic factors for GC patients. Overexpression of miR-601 in AGS and SGC-7901 cells by miR-601 mimic transfection significantly promoted the cell proliferation, migration, and invasion (P < 0.05).

Conclusions: The expression level of miR-601 is dramatically up-regulated in GC. The overexpression of miR-601 promotes the tumor progression of GC, and may be a novel prognostic factor for poor survival in GC patients.

Keywords: Gastric cancer; MicroRNA-601; Prognosis; Progression.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Expression of miR-601 measured by qRT-PCR in GC tissues and cell lines. a MiR-601 expression was upregulated in the GC tissues compared with the adjacent normal controls, which were calculated according to the mean value from 139 GC patients (*** P < 0.001). b The expression of miR-601 was higher in the GC cell lines than that in the normal gastric cells (* P < 0.05, ** P < 0.01 and ***P < 0.001)
Fig. 2
Fig. 2
Kaplan-Meier survival analysis for the GC patients based on the expression of miR-601. Patients with high miR-601 expression had shorter survival time than those with low miR-601 expression (log-rank P = 0.001)
Fig. 3
Fig. 3
Effects of miR-601 on cell proliferation, migration and invasion in AGS and SGC-7901 cells. a In the two cell lines, expression of miR-601 was significantly increased by miR-601 mimic transfection, while transfection with miR-601 inhibitors resulted in lower expression than the corresponding negative control (** P < 0.01 and *** P < 0.001). b Cell proliferation was enhanced by overexpression of miR-601, but was suppressed by knockdown of miR-601 in both AGS and SGC-7901 cells (*P < 0.05 and ** P < 0.01). c and d Overexpression of miR-601 by miR-601 mimic transfection could promote the cell migration and invasion, but the downregulated miR-601 expression could inhibit the cell migration and invasion (**P < 0.01 and ***P < 0.001)
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