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. 2019 Jul 12:6:18.
doi: 10.21037/sci.2019.06.08. eCollection 2019.

Combination of the stromal vascular fraction and platelet-rich plasma accelerates the wound healing process: pre-clinical study in a Sprague-Dawley rat model

Karina  1   2   3 , Muhammad F Samudra  4 , Imam Rosadi  3   5 , Irsyah Afini  3 , Tias Widyastuti  3 , Siti Sobariah  3 , Melinda Remelia  1   6 , Riris L Puspitasari  4 , Iis Rosliana  3 , Tri I Tunggadewi  7
Affiliations

Combination of the stromal vascular fraction and platelet-rich plasma accelerates the wound healing process: pre-clinical study in a Sprague-Dawley rat model

Karina et al. Stem Cell Investig. .

Abstract

Background: Adipose-derived stem cells (ADSCs) improve wound healing owing to their angiogenic potency. However, the production of large quantities of ADSCs for clinical applications is laborious. In this study, the efficacy of the stromal vascular fraction (SVF; non-expanded ADSCs) combined with platelet-rich plasma (PRP) which contains abundant growth factors, for wound healing was evaluated using an animal model.

Methods: PRP from venous blood and SVF from lipoaspirates were harvested from six donors. PRP, SVF, SVF + PRP, and saline solution as a negative control were injected to second degree burn wounds in the backs of 24 male Sprague-Dawley rats. On the seventh day after injection, rats were euthanized and wounds were analyzed microscopically and macroscopically.

Results: Wounds closed faster in the SVF + PRP group than in the control group or PRP or SVF alone groups, with less inflammation, prominent signs of re-epithelization, more skin appendages and blood vessels, and a higher rate of hair growth. No infection or rat death occurred during the trial.

Conclusions: The combination of SVF and PRP may provide an additive stimulatory effect to support angiogenesis and accelerate the wound healing process; accordingly, this combination is a potential alternative to ADSC treatment.

Keywords: Angiogenesis; platelet-rich plasma (PRP); stromal vascular fraction (SVF); wound healing.

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Conflict of interest statement

Conflicts of Interest: The authors have no conflicts of interest to declare.

Figures

Figure 1
Figure 1
Macroscopic (A) and microscopic view (B) immediately following wounding. Hematoxylin-eosin (H&E) stained images were taken by light microscopy at 10× magnification.
Figure 2
Figure 2
Photograph of wound taken at day-7 after injection. Control (A); PRP (B); SVF (C); SVF + PRP (D); hyperemia was found in each group as indicated by prominent rings that delineate wound from non-affected skin (cm scale). SVF, stromal vascular fraction; PRP, platelet-rich plasma.
Figure 3
Figure 3
Mean area of wound measured on post-wound day-7. *, statistically significant with P=0.04; **, statistically significant with P=0.02; ***, statistically significant with P<0.0001, sample size =6, ANOVA-one way with least significant difference post hoc test.
Figure 4
Figure 4
Histopathological examination of day 7 post-burned wound in control group (A), SVF group (B), PRP group (C) and SVF + PRP group (D). Yellow arrow indicates blood vessel and yellow-blue arrow indicates skin appendages (hair follicle and sebaceous gland). Hematoxylin-eosin (H&E) stained image were taken by light microscopy at 10× magnification.
See this image and copyright information in PMC

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