Lymphocyte function-associated antigen-1 (LFA-1) interaction with intercellular adhesion molecule-1 (ICAM-1) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells
- PMID: 3134364
- PMCID: PMC2115164
- DOI: 10.1083/jcb.107.1.321
Lymphocyte function-associated antigen-1 (LFA-1) interaction with intercellular adhesion molecule-1 (ICAM-1) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells
Abstract
Intercellular adhesion molecule-1 (ICAM-1) on the surface of cultured umbilical vein and saphenous vein endothelial cells was upregulated between 2.5- and 40-fold by rIL-1, rTNF, LPS and rIFN gamma corresponding to up to 5 X 10(6) sites/cell. Endothelial cell ICAM-1 was a single band of 90 kD in SDS-PAGE. Purified endothelial cell ICAM-1 reconstituted into liposomes and bound to plastic was an excellent substrate for both JY B lymphoblastoid cell and T lymphoblast adhesion. Adhesion to endothelial cell ICAM-1 in planar membranes was blocked completely by monoclonal antibodies to lymphocyte function associated antigen-1 (LFA-1) or ICAM-1. Adhesion to artificial membranes was most sensitive to ICAM-1 density within the physiological range found on resting and stimulated endothelial cells. Adhesion of JY B lymphoblastoid cells, normal and genetically LFA-1 deficient T lymphoblasts and resting peripheral blood lymphocytes to endothelial cell monolayers was also assayed. In summary, LFA-1 dependent (60-90% of total adhesion) and LFA-1-independent basal adhesion was observed and the use of both adhesion pathways by different interacting cell pairs was increased by monokine or lipopolysaccharide stimulation of endothelial cells. The LFA-1-dependent adhesion could be further subdivided into an LFA-1/ICAM-1-dependent component which was increased by cytokines and a basal LFA-1-dependent, ICAM-1-independent component which did not appear to be affected by cytokines. We conclude that ICAM-1 is a regulated ligand for lymphocyte-endothelial cell adhesion, but at least two other major adhesion pathways exist.
Similar articles
-
Adhesion of T lymphoblasts to epidermal keratinocytes is regulated by interferon gamma and is mediated by intercellular adhesion molecule 1 (ICAM-1).J Exp Med. 1988 Apr 1;167(4):1323-40. doi: 10.1084/jem.167.4.1323. J Exp Med. 1988. PMID: 3128630 Free PMC article.
-
Roles of alpha(4) integrins/VCAM-1 and LFA-1/ICAM-1 in the binding and transendothelial migration of T lymphocytes and T lymphoblasts across high endothelial venules.Int Immunol. 2000 Mar;12(3):241-51. doi: 10.1093/intimm/12.3.241. Int Immunol. 2000. PMID: 10700459
-
Role of LFA-1 and VLA-4 in the adhesion of cloned normal and LFA-1 (CD11/CD18)-deficient T cells to cultured endothelial cells. Indication for a new adhesion pathway.J Immunol. 1992 Feb 15;148(4):1093-101. J Immunol. 1992. PMID: 1371131
-
The CD2-LFA-3 and LFA-1-ICAM pathways: relevance to T-cell recognition.Immunol Today. 1989 Dec;10(12):417-22. doi: 10.1016/0167-5699(89)90039-X. Immunol Today. 1989. PMID: 2482743 Review.
-
Atherosclerosis and inflammation mononuclear cell recruitment and adhesion molecules with reference to the implication of ICAM-1/LFA-1 pathway in atherogenesis.Int J Cardiol. 1998 Oct 1;66 Suppl 1:S45-53; discussion S55. doi: 10.1016/s0167-5273(98)00147-8. Int J Cardiol. 1998. PMID: 9951802 Review.
Cited by
-
Adhesion Molecules in Non-melanoma Skin Cancers: A Comprehensive Review.In Vivo. 2021 May-Jun;35(3):1327-1336. doi: 10.21873/invivo.12385. Epub 2021 Apr 28. In Vivo. 2021. PMID: 33910810 Free PMC article. Review.
-
How endothelial cells regulate transmigration of leukocytes in the inflammatory response.Am J Pathol. 2014 Apr;184(4):886-96. doi: 10.1016/j.ajpath.2013.12.033. Am J Pathol. 2014. PMID: 24655376 Free PMC article.
-
Targeting the β2-integrin LFA-1, reduces adverse neuroimmune actions in neuropathic susceptibility caused by prenatal alcohol exposure.Acta Neuropathol Commun. 2019 Apr 8;7(1):54. doi: 10.1186/s40478-019-0701-y. Acta Neuropathol Commun. 2019. PMID: 30961664 Free PMC article.
-
Monitoring of immunotherapy with cytokines or monoclonal antibodies.Cytotechnology. 1995 Jan;18(1-2):93-106. doi: 10.1007/BF00744324. Cytotechnology. 1995. PMID: 22358641
-
Inhibition of intercellular adhesion molecule 1-dependent biological activities by a synthetic peptide analog.Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2879-82. doi: 10.1073/pnas.88.7.2879. Proc Natl Acad Sci U S A. 1991. PMID: 1672769 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous