Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jun 30;39(6):685-691.
doi: 10.12122/j.issn.1673-4254.2019.06.09.

[Formation of gap junctions between adipose stem cells-derived Schwann cells in a rat model of dyskinesia induced by brain injury]

[Article in Chinese]
Youmeng Yang  1 , Liang Yang  1 , Zhifei Wang  1
Affiliations

[Formation of gap junctions between adipose stem cells-derived Schwann cells in a rat model of dyskinesia induced by brain injury]

[Article in Chinese]
Youmeng Yang et al. Nan Fang Yi Ke Da Xue Xue Bao. .

Abstract
in English, Chinese

Objective: To investigate the formation of gap junctions between Schwann cells derived from differentiated adipose stem cells implanted in a rat model of dyskinesia induced by brain injury and its positive effect in promoting functional recovery of the rats.

Methods: In a rat model of hemiplegia induced by motor cortex injury, adipose stem cells or Schwann cells differentiated from adipose stem cells, either with or without RNAi-mediated silencing of Cx43, were transplanted orthotopically in the lesion. The recovery of the motor function of the rats was observed and scored after the transplantation. Rat brain tissues were sampled to detect the expressions of nerve growth factor (NGF) using Western blotting and RT-PCR.

Results: All the 3 cell transplantation therapies obviously improved the motor function scores of the rats as compared with the control rats. The expression of NGF in the brain tissue was significantly lower in the control group than in the cell transplantation groups. NGF expression in the brain tissues of rats receiving transplantation of Schwann cells with Cx43 gene silencing was lower than that in rats receiving Schwann cells without Cx43 silencing, and was similar with that in rats transplanted with adipose stem cells. The results of RT-PCR showed that NGF mRNA level in the control group was significantly lower than that in the other 3 groups. NGF mRNA expression was the highest in Schwann cell group without Cx43 silencing, followed by adipose stem cell group, and then by Schwann cell group with Cx43 silencing.

Conclusions: In the rat model of dyskinesia induced by brain injury, transplantations of adipose stem cells and adipose stem cells-derived Schwann cells both promote the functional recovery of brain damage, in which gap junction protein Cx43 plays an important role to promote functional gap junction formation possibly by enhancing NGF expression.

目的: 证明移植脂肪干细胞及其诱导分化而来的施旺细胞有利于急性脑损伤动物模型的功能恢复。探索细胞移植后缝隙连接在脑损伤修复过程中的作用机制。

方法: 在运动皮层损伤偏瘫大鼠模型中，将培育分化得到的脂肪干细胞及施旺细胞以及RNAi技术沉默Cx43基因的施旺细胞移植损伤灶处行原位培养，术后观察大鼠运动功能恢复情况并予评分。移植7 d后处死取鼠脑组织，RT-PCR和Western blot验证神经生长因子（NGF）的表达水平。

结果: 在建立的运动皮层损伤的偏瘫大鼠模型，细胞移植组大鼠优于对照组运动评分。WB结果中对照组NGF表达明显低于各移植组，Cx43基因沉默的施旺细胞移植后组织NGF表达低于未沉默组，Cx43基因未沉默组与脂肪干细胞移植组无显著性差异。RT-PCR法测得对照组NGF mRNA水平明显低于各移植组，施旺细胞组水平高于脂肪干细胞组、高于Cx43基因沉默施旺细胞组。

结论: 移植脂肪干细胞和其经诱导分化而来的施旺细胞，都有利于动物模型的脑损伤后功能恢复。在脑损伤修复期功能性缝隙连接形成过程中，缝隙连接蛋白Cx43发挥了重要作用，可能通过促进神经生长因子NGF的表达有关。

Keywords: Cx43; Schwann cells; adipose stem cells; gap junctions; nerve growth factor; traumatic brain injury.

PubMed Disclaimer

Figures

1
1
移植用ADSC-SC细胞 Induction of ADSC-SCs and identification of Cx43 expression. 1: ADSC-SCs with RNAi for Cx43, under light microscope (A) and after fluorescence immunocytochemistry (B). 2: ADSC-SCs with RNAi for NC, under light microscope (C) and after fluorescence immunocytochemistry (D) (Original magnification: ×400).
2
2
移植前细胞Cx43表达WB结果：ADSC细胞经NC对照组慢病毒转染与Cx43沉默慢病毒转染后Cx43表达差异 Expression of Cx43 in ADSCs and ADSC-SCs transfected with RNAi-NC and RNAi-Cx43. The level of Cx43 in ADSCsSCs with RNAi for Cx43 group is significantly lower than both ADSCs-SCs group and ADSCs- SCs with RNAi for NC control group. *P < 0.05 vs control group.
3
3
Western blot法测定各组NGF表达水平 Determination of NGF expression levels in each group after cell transplantation by Western blotting. 1: Control group by saline. 2: Transplantation with ADSCs; 3: Transplantation with RNAi-NC transfectioned ADSCs-SCs; 4: Transplantation with RNAi-Cx43 transfectioned ADSCs-SCs. **P < 0.00 vs control group.
4
4
反应产物熔点曲线 Melting point curve of reaction product.
5
5
各组目的基因mRNA相对含量 Relative mRNA levels of target genes in each group. 1: Con; 2: ADSCs; 3: ADSCs-SCs+RNAi-NC; 4: ADSCs-SCs+RNAi-Cx43. All the parameters had difference(P < 0.05) among multi-group.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Ghobrial GM, Amenta PS, Maltenfort MA, et al. Longitudinal incidence and concurrence rates for traumatic brain injury and spine injury - A twenty year analysis. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=11c218c59.... Clin Neurol Neurosurg. 2014;123(8):174–80. - PubMed
    1. Moorin R, Miller TR, Hendrie D. Population-based incidence and 5-year survival for hospital-admitted traumatic brain and spinal cord injury, Western Australia, 2003-2008. J Neurol. 2014;261(9):1726–34. doi: 10.1007/s00415-014-7411-y. - DOI - PubMed
    1. Schwab ME, Bartholdi D. Degeneration and regeneration of axons in the lesioned spinal cord. Physiol Rev. 1996;76(2):319–70. doi: 10.1152/physrev.1996.76.2.319. - DOI - PubMed

    1. Kuscha, Veronika. Cellular and axonal plasticity in the lesioned spinal cord of adult zebrafish[D]. Edinburgh: University of Edinburgh, 2011.

    1. Hong-Shi F, Yan W, Guo-Ping C. Microenvironment for axonal regeneration after peripheral nerve injury(review) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgkfllysj... Chin J Rehabil Theory Pract. 2015;(3):288–91.

Grants and funding

国家自然科学基金青年科学基金（81401006）；湖南省自然科学基金（12JJ5045）

LinkOut - more resources