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. 2019 Jul 2;11(7):387.
doi: 10.3390/toxins11070387.

Fumonisin B1 Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium Cells

Qiaoling Yuan  1 , Yancheng Jiang  2 , Ying Fan  2 , Yingfeng Ma  2 , Hongyu Lei  3 , Jianming Su  4
Affiliations

Fumonisin B1 Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium Cells

Qiaoling Yuan et al. Toxins (Basel). .

Abstract

Fumonisins (Fums) are types of mycotoxin that widely contaminante feed material crops, and can trigger potential biological toxicities to humans and various animals. However, the toxicity of Fums on porcine blood vessels has not been fully explored. Fumonisin B1 (FB1) is the main component of Fums. Therefore, the aim of this study was to explore the effects of FB1 on the oxidative stress and tight junctions of the pig iliac endothelial cells (PIECs) in vitro. The results showed that FB1 reduced the viability of PIECs, increased the contents of lipid peroxidation product malondialdehyde (MDA), decreased the activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and thioredoxin reductase (TrxR), and decreased the level of glutathione (GSH). In addition, the barrier functions were destroyed, along with the down-regulations on Claudin 1, Occludin and ZO-1 and the increase of paracellular permeability. Thus, this research indicates that FB1 facilitates oxidative stress and breaks barrier functions to damage pig iliac endothelium cells.

Keywords: Fumonisin B1; oxidative stress; pig iliac endothelial cells; tight junction.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The effect of FB1 treatment on cell viability of PIECs. The cell viability of PIECs exposed to different concentrations of FB1 for 24 h (a); The cell viability of PIECs exposed different concentrations of FB1 for 48 h (b); The cell viability of PIECs exposed different concentrations of FB1 for 72 h (c); The cell viability curve of PIECs exposed to FB1 for 72 h (d). The values were represented as ± standard deviation (SD), * p < 0.05, compared with control group.
Figure 1
Figure 1
The effect of FB1 treatment on cell viability of PIECs. The cell viability of PIECs exposed to different concentrations of FB1 for 24 h (a); The cell viability of PIECs exposed different concentrations of FB1 for 48 h (b); The cell viability of PIECs exposed different concentrations of FB1 for 72 h (c); The cell viability curve of PIECs exposed to FB1 for 72 h (d). The values were represented as ± standard deviation (SD), * p < 0.05, compared with control group.
Figure 2
Figure 2
The effect of FB1 treatment for 48 h on oxidative stress of PIECs. The oxidation indices of MDA (a), SOD (b), GSH (c), GSH-Px (d), CAT (e) and TrxR (f). Data were represented as means ± SD, * p < 0.05, compared with control group.
Figure 3
Figure 3
FB1 increased permeability of PIECs. The endothelial permeability was determined by the Transwell assay. The values were represented as means ± SD, * p < 0.05, compared with control group.
Figure 4
Figure 4
FB1 restrained the expressions of Claudin 1, Occludin, and ZO-1 in PIECs. The quantitative qPCR and western blot analysis of Claudin 1 (a,d), Occludin (b,e), and ZO-1 (c,f) were performed. In western blot analysis, the glyceraldehyde phosphate dehydrogenase (GAPDH) served as the internal reference control. The values were represented as means ± SD, * p < 0.05, compared with control group.
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