Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 May;138(2):505-9.
doi: 10.1128/jb.138.2.505-509.1979.

Characterization of a site-specific restriction endonuclease from Rhodopseudomonas sphaeroides

S P Lynn, L K Cohen, J F Gardner, S Kaplan

Characterization of a site-specific restriction endonuclease from Rhodopseudomonas sphaeroides

S P Lynn et al. J Bacteriol. 1979 May.

Abstract

A type II restriction endonuclease, RshI, has been partially purified from photoheterotrophically grown Rhodopseudomonas sphaeroides strain 2.4.1. The enzyme preparation, after a single DE-52 column fractionation, is free of 5' exonuclease and phosphatase activities but contains a trace of 3' exonuclease activity. Based upon deoxyribonucleic acid (DNA) sequencing data in the vicinity of the enzyme-promoted cleavage of pBR322 DNA, we have concluded that RshI probably recognizes the palinodromic hexanucleotide sequence 5'-CGATCG-3' and cleaves between the T and C. lambda cI857 DNA contains three RshI sites, two of which lie in the replaceable region. The plasmid pBR322, which carries resistances to ampicillin and tetracycline, contains a single RshI site in the ampicillin resistance determinant. Insertion of DNA into the RshI site of pBR322 results in loss of ampicillin resistance but retention of tetracycline resistance, thereby providing a convenient screening procedure for recombinant plasmids.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Science. 1978 May 5;200(4341):494-502 - PubMed
    1. J Mol Biol. 1978 Dec 5;126(2):241-58 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737-41 - PubMed
    1. J Biol Chem. 1978 Jan 25;253(2):451-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 - PubMed

Publication types

MeSH terms

LinkOut - more resources