Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1987 May;84(10):3107-11.
doi: 10.1073/pnas.84.10.3107.

Deduced amino acid sequence of bovine retinal Go alpha: similarities to other guanine nucleotide-binding proteins

K P Van Meurs, C W Angus, S Lavu, H F Kung, S K Czarnecki, J Moss, M Vaughan
Comparative Study

Deduced amino acid sequence of bovine retinal Go alpha: similarities to other guanine nucleotide-binding proteins

K P Van Meurs et al. Proc Natl Acad Sci U S A. 1987 May.

Abstract

A bovine retinal cDNA clone encoding the complete sequence (354 amino acids) of Go alpha, a guanine nucleotide-binding protein (G protein), was isolated by using oligonucleotide probes complementary to published sequences in two putative clones for the alpha subunit of bovine transducin (T alpha). The deduced amino acid sequence contained sequences identical to those in seven tryptic peptides (total 63 amino acids) from bovine brain Go alpha. The cDNA for bovine retinal Go alpha exhibits greater than 90% identity in both coding and 3' untranslated regions with a recently described partial cDNA clone for Go alpha from rat brain [Itoh, H., Kozasa, T., Nagata, S., Nakamura, S., Katada, T., Ui, M., Iwai, S., Ohtsuka, E., Kawasaki, H., Suzuki, K. & Kaziro, Y. (1986) Proc. Natl. Acad. Sci. USA 83, 3776-3780]. Comparison of the nucleotide and deduced amino acid sequences of the bovine Go alpha clone with those previously reported for other G proteins of bovine origin (Gs alpha, Gi alpha, and T alpha) reveals extensive regions identical to those surrounding the amino acids modified by cholera toxin and pertussis toxin. There are also marked similarities of sequence in regions of the G proteins, elongation factors, and the ras p21 gene products that are believed to be involved in guanine nucleotide binding and GTP hydrolysis.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Methods Enzymol. 1980;65(1):499-560 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6516-20 - PubMed
    1. J Biol Chem. 1981 Aug 10;256(15):8102-9 - PubMed
    1. J Biol Chem. 1982 Sep 25;257(18):10540-3 - PubMed

Publication types

Associated data

LinkOut - more resources