Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Mar 26;20(3):705-710.
doi: 10.31557/APJCP.2019.20.3.705.

Hypoxia-Inducible Factor1-Α (HIF1α) and Vascular Endothelial Growth Factor-A (VEGF-A) Expression in De Novo AML Patients

Mohammad Jabari  1 , Mehdi Allahbakhshian Farsani  1   2 , Sina Salari  2 , Mohsen Hamidpour  1 , Vahid Amiri  1 , Mohammad Hossein Mohammadi  1   2
Affiliations

Hypoxia-Inducible Factor1-Α (HIF1α) and Vascular Endothelial Growth Factor-A (VEGF-A) Expression in De Novo AML Patients

Mohammad Jabari et al. Asian Pac J Cancer Prev. .

Abstract

Background: Bone marrow hypoxia can promote leukemia progression in human cases of acute myeloid leukemia (AML). In addition, low oxygen tension is able to regulate the expression of different genes involved in malignancy. In this study, we hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF-A) genes were assessed as principal regulators of hypoxia in do novo AML patients. Methods: Peripheral blood and bone marrow samples were collected from 57 AML patients and 17 normal control subjects with informed consent. Expression of HIF1α and VEGF-A was then evaluated using quantitative real-time PCR (Q-Real time PCR) and data were analyzed with SPSS 16. Result: HIF1α and VEGF-A showed overexpression in AML patients compared to normal controls (P <0.0001 and P<0.005, respectively). The expression level of HIF1α was significantly higher in AML-M3 cases versus AML-non M3 cases. Furthermore, there was a positive correlation between HIF1α and VEGF-A ( P <0.0001 and r = 0.497). Conclusion: Adding to the many studies on the role of hypoxia in solid tumors, our data indicate that HIF1a and VEGF-A overexpression also occurs in AML patients. We consider that this is possibly involved in leukemic cell growth and therefore could be a promising target for clinical control.

Keywords: Acute myeloid leukemia; hypoxia; HIF1α; VEGF.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Relative Expression of HIF1α and VEGF-A in 57 AML Patients and 17 Healthy Patients was Measured from Ct Values and Normalized against a Reference Gene (ABL). A) A significant difference (P < 0.0001) between HIF1α expression in AML patients and healthy patients were identified. A relative HIF1α expression level of 12.61 ± 3.25 (SD) was measured in AML patients in comparison to 2.46 ± 0.33 (SD) in the normal control group. B) A significant difference (P < 0.005) between VEGF-A expression in AML patients and healthy patients were also identified. A relative VEGF-A expression level of 5.04 ± 0.76 (SD) was measured in AML patients in comparison to 2.003 ± 0.24 (SD) in the normal control group
Figure 2
Figure 2
Expression of HIF1α and VEGF Genes in Patients with APL
Figure 3
Figure 3
Statistical Analysis by Means of Spearman test Reveals Dependence and Relation between the Expression of HIF1α and VEGF-A. A) Correlation between HIF1a and VEGF-A in 57 AML patients was determined to be positive and significant (P <0.0001 r = 0.497
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Aguayo A, Kantarjian HM, Estey EH, et al. Plasma vascular endothelial growth factor levels have prognostic significance in patients with acute myeloid leukemia but not in patients with myelodysplastic syndromes. Cancer. 2002;95:1923–30. - PubMed
    1. Albitar M. Angiogenesis in acute myeloid leukemia and myelodysplastic syndrome. Acta Haematol. 2001;106:170–6. - PubMed
    1. Ayala F, Dewar R, Kieran M, et al. Contribution of bone microenvironment to leukemogenesis and leukemia progression. Leukemia. 2009;23:2233–41. - PMC - PubMed
    1. Bangoura G, Liu ZS, Qian Q, et al. Prognostic significance of HIF-2alpha/EPAS1 expression in hepatocellular carcinoma. World J Gastroenterol. 2007;13:3176–82. - PMC - PubMed
    1. Bellamy WT, Richter L, Frutiger Y, et al. Expression of vascular endothelial growth factor and its receptors in hematopoietic malignancies. Cancer Res. 1999;59:728–33. - PubMed

MeSH terms

Substances