Fluorescence in situ hybridization in plants: recent developments and future applications

doi:10.1007/s10577-019-09607-z

Review

. 2019 Sep;27(3):153-165.

doi: 10.1007/s10577-019-09607-z. Epub 2019 Mar 9.

Fluorescence in situ hybridization in plants: recent developments and future applications

Jiming Jiang¹

Affiliations

PMID: 30852707
DOI: 10.1007/s10577-019-09607-z

Review

Fluorescence in situ hybridization in plants: recent developments and future applications

Jiming Jiang. Chromosome Res. 2019 Sep.

. 2019 Sep;27(3):153-165.

doi: 10.1007/s10577-019-09607-z. Epub 2019 Mar 9.

Author

Jiming Jiang¹

Affiliation

¹ Department of Plant Biology, Department of Horticulture, Michigan State University, East Lansing, MI, 48824, USA. jiangjm@msu.edu.

PMID: 30852707
DOI: 10.1007/s10577-019-09607-z

Abstract

Fluorescence in situ hybridization (FISH) was developed more than 30 years ago and has been the most paradigm-changing technique in cytogenetic research. FISH has been used to answer questions related to structure, mutation, and evolution of not only individual chromosomes but also entire genomes. FISH has served as an important tool for chromosome identification in many plant species. This review intends to summarize and discuss key technical development and applications of FISH in plants since 2006. The most significant recent advance of FISH is the development and application of probes based on synthetic oligonucleotides (oligos). Oligos specific to a repetitive DNA sequence, to a specific chromosomal region, or to an entire chromosome can be computationally identified, synthesized in parallel, and fluorescently labeled. Oligo probes designed from conserved DNA sequences from one species can be used among genetically related species, allowing comparative cytogenetic mapping of these species. The advances with synthetic oligo probes will significantly expand the applications of FISH especially in non-model plant species. Recent achievements and future applications of FISH and oligo-FISH are discussed.

Keywords: Chromosome; Evolution; FISH; Genome; Karyotype; Oligo-FISH.

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References

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[1] PLoS One. 2014 Dec 10;9(12):e115108 - PubMed

[2] PLoS One. 2014 Dec 10;9(12):e115108 - PubMed

[3] Genome. 2006 Sep;49(9):1057-68 - PubMed

[4] Genome. 2006 Sep;49(9):1057-68 - PubMed

[5] Front Plant Sci. 2018 Nov 06;9:1624 - PubMed

[6] Front Plant Sci. 2018 Nov 06;9:1624 - PubMed

[7] Mol Cytogenet. 2015 Oct 24;8:80 - PubMed

[8] Mol Cytogenet. 2015 Oct 24;8:80 - PubMed

[9] Plant J. 2006 Sep;47(6):977-86 - PubMed

[10] Plant J. 2006 Sep;47(6):977-86 - PubMed

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Fluorescence in situ hybridization in plants: recent developments and future applications

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Fluorescence in situ hybridization in plants: recent developments and future applications

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