Skip to main page content
U.S. flag

An official website of the United States government

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jan 9:9:1895.
doi: 10.3389/fphys.2018.01895. eCollection 2018.

Different Transcriptomic Responses to Thermal Stress in Heat-Tolerant and Heat-Sensitive Pacific Abalones Indicated by Cardiac Performance

Nan Chen  1   2 , Zekun Huang  1   3   4 , Chengkuan Lu  3   4 , Yawei Shen  1   3   4 , Xuan Luo  1   3   4 , Caihuan Ke  1   3   4 , Weiwei You  1   3   4
Affiliations

Different Transcriptomic Responses to Thermal Stress in Heat-Tolerant and Heat-Sensitive Pacific Abalones Indicated by Cardiac Performance

Nan Chen et al. Front Physiol. .

Abstract

The Pacific abalone Haliotis discus hannai is one of the most economically important mollusks in China. Even though it has been farmed in southern China for almost 20 years, summer mortality remains the most challengeable problem for Pacific abalone aquaculture recently. Here, we determined the different heat tolerance ability for five selective lines of H. discus hannai by measuring the cardiac performance and Arrhenius breakpoint temperature (ABT). The Red line (RL) and Yangxia line (YL) were determined as the most heat-sensitive and most heat-tolerant line, respectively. Heart rates for RL were significantly lower than those of the YL at the same temperature (p < 0.05). The differentially expressed genes (DEGs), which were enriched in several pathways including cardiac muscle contraction, glutathione metabolism and oxidative phosphorylation, were identified between RL and YL at control temperature (20°C) and heat stress temperature (28.5°C, the ABT of the RL) by RNA-seq method. In the RL, 3370 DEGs were identified between the control and the heat-stress temperature, while only 1351 DEGs were identified in YL between these two temperature tests. Most of these DEGs were enriched in the pathways such as protein processing in endoplasmic reticulum, nucleotide binding and oligomerization domain (NOD) like receptor signaling, and ubiquitin mediated proteolysis. Notably, the most heat-tolerant line YL used an effective heat-protection strategy based on moderate transcriptional changes and regulation on the expression of key genes.

Keywords: ABT; Pacific abalone; cardiac performance; heat stress; transcriptome.

PubMed Disclaimer

Figures

FIGURE 1
FIGURE 1
The Arrhenius breakpoint temperature (ABT) for different Pacific abalone lines. (A) The Arrhenius plots for YL and RL. (B) The ABTs for the five selective lines. Bars labeled with different letters are significantly different (p < 0.05).
FIGURE 2
FIGURE 2
The Venn diagram of significant differently expressed genes. (A) Comparison between different lines. (B) Comparison between control temperature and heat stress temperature. C_RL: RL at control temperature; C_YL: YL at control temperature; H_RL: RL at heat stress temperature; H_YL: YL at heat stress temperature. Up-regulated genes were numbered in red and down-regulated genes were numbered in black.
FIGURE 3
FIGURE 3
Top 30 GO terms enrichment statistics between RL and YL at control temperature.
FIGURE 4
FIGURE 4
Top 30 GO terms enrichment statistics between control and heat stress temperature. (A) Terms enrichment in RL. (B) Terms enrichment in YL.
FIGURE 5
FIGURE 5
Top 10 pathways enrichment statistics between RL and YL at control temperature.
FIGURE 6
FIGURE 6
Top 30 pathways enrichment statistics between control and heat stress temperature. (A) Pathway enrichment in the RL. (B) Pathway enrichment in the YL.
FIGURE 7
FIGURE 7
Heatmaps of differentially expressed heat shock protein (HSP) genes. (A) Genes differentially expressed in both lines. (B) Genes differentially expressed in the RL only. (C) Genes differentially expressed in the YL only.
FIGURE 8
FIGURE 8
Heatmap of genes involved in the ubiquitin-mediated proteolysis pathway.
FIGURE 9
FIGURE 9
Heatmap of differentially expressed antioxidant-related genes. CAT, catalase; SOD, superoxide dismutase; PRDX5, peroxiredoxin 5.
FIGURE 10
FIGURE 10
Comparison of qRT-PCR and RNA-seq results. (A) H_RL vs. C_RL; (B) H_YL vs. C_YL.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Abele D., Heise K., Portner H. O., Puntarulo S. (2002). Temperature-dependence of mitochondrial function and production of reactive oxygen species in the intertidal mud clam Mya arenaria. J. Exp. Biol. 205 1831–1841. - PubMed
    1. Alter K., Andrewartha S. J., Morash A. J., Clark T. D., Hellicar A. D., Leon R. I., et al. (2017). Hybrid abalone are more robust to multi-stressor environments than pure parental species. Aquaculture 478 25–34. 10.1016/j.aquaculture.2017.04.035 - DOI
    1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. (1990). Basic local alignment search tool. J. Mol. Biol. 215 403–410. 10.1016/S0022-2836(05)80360-2 - DOI - PubMed
    1. Anders S., Huber W. (2012). Differential Expression of RNA-Seq Data at the Gene Level–the DESeq Package. Heidelberg: European Molecular Biology Laboratory (EMBL).
    1. Barshis D. J., Ladner J. T., Oliver T. A., Seneca F. O., Traylor-Knowles N., Palumbi S. R. (2013). Genomic basis for coral resilience to climate change. Proc. Natl. Acad. Sci. U.S.A. 110 1387–1392. 10.1073/pnas.1210224110 - DOI - PMC - PubMed

LinkOut - more resources