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Review
. 2019 Jan;21(1):72-84.
doi: 10.1038/s41556-018-0251-8. Epub 2019 Jan 2.

Super-resolution microscopy demystified

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Review

Super-resolution microscopy demystified

Lothar Schermelleh et al. Nat Cell Biol. 2019 Jan.

Abstract

Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.

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