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. 2019 Jan:228:53-60.
doi: 10.1016/j.vetmic.2018.11.013. Epub 2018 Nov 19.

Hemagglutinin-Neuraminidase and fusion genes are determinants of NDV thermostability

Tong Liu  1 , Yang Song  1 , Yanling Yang  1 , Yawen Bu  1 , Jinlong Cheng  1 , Guozhong Zhang  1 , Jia Xue  2
Affiliations

Hemagglutinin-Neuraminidase and fusion genes are determinants of NDV thermostability

Tong Liu et al. Vet Microbiol. 2019 Jan.

Abstract

Newcastle disease (ND) caused by infections with virulent strains of Newcastle disease virus (NDV) continues to be a threat for poultry industry worldwide. The prospect of developing a thermostable and effective NDV vaccine is still highly desirable. To investigate the determinants of thermostability in NDV, we generated recombinant NDV strains by exchanging viral hemagglutinin-neuraminidase (HN) gene or by mutating the fusion (F) gene. The results showed that the HN and F protein were both determinants of NDV thermostability. With increased thermostability, the HN protein-chimeric virus showed significantly reduced neuraminidase and hemadsorption activities, but its hemolytic activity was retained. We also found that changing the amino acid in the F protein cleavage sites, affected the thermostability as well as the pathogenicity and fusogenic capacity of the virus. Taken together, our results suggest that HN and F proteins both contribute to the thermostability of NDV, and other viral biological activities change as the thermostability of the virus changes. These findings should be of benefit to the development of a thermostable and efficacious NDV vaccine.

Keywords: Biological activity; Genotype VII; Newcastle disease virus; Thermostability.

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