Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Nov 9;10(11):465.
doi: 10.3390/toxins10110465.

Dose and Exposure Time-Dependent Renal and Hepatic Effects of Intraperitoneally Administered Fumonisin B₁ in Rats

András Szabó  1   2   3 , Judit Szabó-Fodor  4 , Mariam Kachlek  5 , Miklós Mézes  6   7 , Krisztián Balogh  8   9 , Róbert Glávits  10 , Omeralfaroug Ali  11 , Yarsmin Yunus Zeebone  12 , Melinda Kovács  13   14
Affiliations

Dose and Exposure Time-Dependent Renal and Hepatic Effects of Intraperitoneally Administered Fumonisin B₁ in Rats

András Szabó et al. Toxins (Basel). .

Abstract

Male Wistar rats were treated intraperitoneally (i.p.) with fumonisin B₁ (FB₁; 0, 20, 50 and 100 mg/kg dietary dose equivalent) for 5 and 10 days (n = 24⁻24 in each setting) to gain dose- and time-dependent effects on antioxidant status and oxidative stress response, clinical chemical endpoints and liver, kidney and lung histopathology and lymphocyte damage (genotoxicity). FB₁ decreased feed intake, body weight gain and absolute liver weight, irrespective of the toxin dose. Relative kidney weight increased in the 10-day setting. Linear dose response was found for plasma aspartate aminotransferase, alanine aminotransferase, total cholesterol, urea and creatinine, and exposure time-dependence for plasma creatinine level. The latter was coupled with renal histopathological findings, tubular degeneration and necrosis and the detachment of tubular epithelial cells. The pronounced antioxidant response (reduced glutathione accretion, increasing glutathione peroxidase activity) referred to renal cortical response (5⁻10 days exposure at 50⁻100 ppm FB₁). Hepatic alterations were moderate, referring to initial phase lipid peroxidation (exposure time dependent difference of conjugated diene and triene concentrations), and slight functional disturbance (↑ total cholesterol). Lymphocyte DNA damage was moderate, supporting a mild genotoxic effect of FB₁.

Keywords: dose-dependence; fumonisin B1; genotoxicity; hepatotoxicity; nephrotoxicity; rat.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Vacuolar degeneration of the hepatocytes (arrow) after 100 ppm fumonisin B1 exposure in the 10-day experiment (hematoxylin-eosin, 400×).
Figure 2
Figure 2
Solitaire necrosis and formation of Councilman bodies (arrow) in the liver of rats after 100 ppm fumonisin B1 exposure in the 10-day experiment (hematoxylin-eosin, 400×).
Figure 3
Figure 3
Necrotic and detached tubular epithelial cells in the tubular lumen (arrow) of the kidney after 100 ppm fumonisin B1 exposure in the 10-day experiment (hematoxylin-eosin, 200×).
Figure 4
Figure 4
Tubular dilatation (arrow) in the kidney of rats after 100 ppm fumonisin B1 exposure in the 10-day experiment (hematoxylin-eosin, 200×).
Figure 5
Figure 5
Log tail intensity values (median ± SD; n = 6/group) corresponding to DNA damage of lymphocytes after 5 and 10 days of intraperitoneal exposure of animals to increasing concentrations of FB1 (C: control, 20, 50, 100 mg/kg dietary dose equivalent).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Rheeder J.P., Marasas W.F.O., Vismer H.F. Production of fumonisin analogs by Fusarium species. Appl. Environ. Microbiol. 2002;68:2101–2105. doi: 10.1128/AEM.68.5.2101-2105.2002. - DOI - PMC - PubMed
    1. European Commission (2003) Updated opinion of the Scientific Committee on Food (SCF) on Fumonisin B1, BIOMIN Mycotoxin Survey, (2017): BIOMIN Holding GmbH, Getzersdorf, Austria. [(accessed on 12 October 2018.)]; Available online: https://info.biomin.net/acton/attachment/14109/f-0463/1/-/-/l-0009/l-000....
    1. Kubena L., Edrington T., Harvey R., Buckley S., Phillips T., Rottinghaus G., Casper H. Individual and combined effects of fumonisin B1 present in Fusarium moniliforme culture material and T-2 toxin or deoxynivalenol in broiler chicks. Poult. Sci. 1997;76:1239–1247. doi: 10.1093/ps/76.9.1239. - DOI - PubMed
    1. Månsson M., Klejnstrup M.L., Phipps R.K., Nielsen K.F., Frisvad J.C., Gotfredsen C.H., Larsen T.O. Isolation and NMR characterization of fumonisin B2 and a new fumonisin B6 from Aspergillus niger. J. Agric. Food Chem. 2010;58:949–953. doi: 10.1021/jf902834g. - DOI - PubMed
    1. Candlish A., Aidoo K., Smith J., Pearson S. A limited survey of aflatoxins and fumonisins in retail maizebased products in the UK using immunoassay detection. Mycotoxin Res. 2000;16:2–8. doi: 10.1007/BF02946100. - DOI - PubMed

MeSH terms

LinkOut - more resources