Specificity of bacteriophage Mu excision
- PMID: 3039296
- DOI: 10.1007/BF00331606
Specificity of bacteriophage Mu excision
Abstract
To study the excision of bacteriophage Mu at the DNA sequence level, the Mu-derived phage lambda placMu3 was transposed to the transcribed but non-translated leader region of a plasmid-borne tetracycline (tet) resistance gene. Revertants (excision products) were then selected by Tet+ restoration of Tet+ and characterized. Of 21 independent Tet+ revertants, 17 contained simple deletions of most or all of lambda placMu3, while the other four contained more complex rearrangements in which one end of lambda placMu3 had been transposed, and most of the prophage had been deleted. The deletion endpoints were found in short direct repeats in each of the complex rearrangements and in 11 of the 17 simple deletion excisants. The results suggest models of slipped mispairing of template and nascent DNA strands facilitated by proteins of the Mu transposition machinery.
Similar articles
-
Lambda placMu: a transposable derivative of bacteriophage lambda for creating lacZ protein fusions in a single step.J Bacteriol. 1984 Jun;158(3):1084-93. doi: 10.1128/jb.158.3.1084-1093.1984. J Bacteriol. 1984. PMID: 6327627 Free PMC article.
-
[Transposition immunity in bacteriophage Mu. The effect of a mutation at the kil gene on the establishment of immunity].Genetika. 1985 Jun;21(6):927-35. Genetika. 1985. PMID: 2993102 Russian.
-
Transposition of lambda placMu is mediated by the A protein altered at its carboxy-terminal end.Gene. 1988 Nov 15;71(1):177-86. doi: 10.1016/0378-1119(88)90089-3. Gene. 1988. PMID: 2850974
-
Mechanism of transposition of bacteriophage Mu: polarity of the strand transfer reaction at the initiation of transposition.Cell. 1984 Dec;39(2 Pt 1):395-404. doi: 10.1016/0092-8674(84)90018-7. Cell. 1984. PMID: 6094017
-
Genetic map of bacteriophage lambda.Microbiol Rev. 1978 Sep;42(3):577-91. doi: 10.1128/mr.42.3.577-591.1978. Microbiol Rev. 1978. PMID: 362150 Free PMC article. Review. No abstract available.
Cited by
-
The right end of MudI(Ap,lac).Arch Microbiol. 1989;153(1):1-6. doi: 10.1007/BF00277532. Arch Microbiol. 1989. PMID: 2532881
-
The use of lambda plac-Mu hybrid phages in Klebsiella pneumoniae and the isolation of stable Hfr strains.Mol Gen Genet. 1989 Feb;215(3):529-36. doi: 10.1007/BF00427052. Mol Gen Genet. 1989. PMID: 2540416
-
Two copies of a DNA element, 'Wendy', in the chloroplast chromosome of Chlamydomonas reinhardtii between rearranged gene clusters.Plant Mol Biol. 1995 Oct;29(1):63-80. doi: 10.1007/BF00019119. Plant Mol Biol. 1995. PMID: 7579168
-
DNA rearrangement mediated by inverted repeats.Proc Natl Acad Sci U S A. 1996 Jan 23;93(2):819-23. doi: 10.1073/pnas.93.2.819. Proc Natl Acad Sci U S A. 1996. PMID: 8570641 Free PMC article.
-
Identification of phosphate starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu d1) transcriptional fusions.J Bacteriol. 1990 Jun;172(6):3191-200. doi: 10.1128/jb.172.6.3191-3200.1990. J Bacteriol. 1990. PMID: 2160940 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Research Materials
