doi: 10.1002/j.1460-2075.1987.tb02375.x.
Interaction of the TGGCA-binding protein with upstream sequences is required for efficient transcription of mouse mammary tumor virus
- PMID: 3038519
- PMCID: PMC553940
- DOI: 10.1002/j.1460-2075.1987.tb02375.x
Item in Clipboard
Interaction of the TGGCA-binding protein with upstream sequences is required for efficient transcription of mouse mammary tumor virus
EMBO J.
1987 May.
Abstract
A high-affinity binding site for the TGGCA-binding protein, also known as nuclear factor I, has previously been shown to reside within the mouse mammary tumor virus (MMTV) long terminal repeat. We have introduced mutations into this binding site to test the importance of this ubiquitous nuclear protein in MMTV transcription. Mutations which abolish the binding of the TGGCA protein in vitro are shown to impair strongly glucocorticoid-induced transcription from this promoter in vivo. These data demonstrate that the TGGCA-binding protein is a multifunctional DNA-binding protein, capable of serving a transcriptional role in the case of MMTV, in addition to its known involvement in the replication of adenovirus.
Similar articles
-
Nuclear factor I acts as a transcription factor on the MMTV promoter but competes with steroid hormone receptors for DNA binding.EMBO J. 1990 Jul;9(7):2233-9. doi: 10.1002/j.1460-2075.1990.tb07393.x. EMBO J. 1990. PMID: 2162764 Free PMC article.
-
The TGGCA protein binds to the MMTV-LTR, the adenovirus origin of replication, and the BK virus enhancer.Nucleic Acids Res. 1985 Mar 25;13(6):2045-61. doi: 10.1093/nar/13.6.2045. Nucleic Acids Res. 1985. PMID: 2987840 Free PMC article.
-
Interplay of steroid hormone receptors and transcription factors on the mouse mammary tumor virus promoter.J Steroid Biochem Mol Biol. 1992 Oct;43(5):365-78. doi: 10.1016/0960-0760(92)90071-p. J Steroid Biochem Mol Biol. 1992. PMID: 1327070 Review.
-
Constitutive repression and nuclear factor I-dependent hormone activation of the mouse mammary tumor virus promoter in Saccharomyces cerevisiae.Mol Cell Biol. 1995 Dec;15(12):6987-98. doi: 10.1128/MCB.15.12.6987. Mol Cell Biol. 1995. PMID: 8524266 Free PMC article.
-
Transcriptional control by steroid hormones: the role of chromatin.Ciba Found Symp. 1995;191:7-17; discussion 17-23. doi: 10.1002/9780470514757.ch2. Ciba Found Symp. 1995. PMID: 8582206 Review.
Cited by
-
DNA rotational positioning in a regulatory nucleosome is determined by base sequence. An algorithm to model the preferred superhelix.Nucleic Acids Res. 1990 Dec 11;18(23):6981-7. doi: 10.1093/nar/18.23.6981. Nucleic Acids Res. 1990. PMID: 2175885 Free PMC article.
-
DNA sequences outside the receptor-binding sites differently modulate the responsiveness of the mouse mammary tumour virus promoter to various steroid hormones.EMBO J. 1988 May;7(5):1403-10. doi: 10.1002/j.1460-2075.1988.tb02957.x. EMBO J. 1988. PMID: 2842149 Free PMC article.
-
A progesterone responsive element maps to the far upstream steroid dependent DNase hypersensitive site of chicken lysozyme chromatin.EMBO J. 1988 Jul;7(7):2063-73. doi: 10.1002/j.1460-2075.1988.tb03046.x. EMBO J. 1988. PMID: 3416833 Free PMC article.
-
Numerous nuclear proteins bind the long control region of human papillomavirus type 16: a subset of 6 of 23 DNase I-protected segments coincides with the location of the cell-type-specific enhancer.J Virol. 1989 Mar;63(3):1142-52. doi: 10.1128/JVI.63.3.1142-1152.1989. J Virol. 1989. PMID: 2536825 Free PMC article.
-
Dual function of a nuclear factor I binding site in MMTV transcription regulation.Nucleic Acids Res. 1989 Apr 25;17(8):3065-78. doi: 10.1093/nar/17.8.3065. Nucleic Acids Res. 1989. PMID: 2542892 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
