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. 2019 Jan 8;47(D1):D971-D975.
doi: 10.1093/nar/gky1045.

DSMNC: a database of somatic mutations in normal cells

Xuexia Miao  1 , Xi Li  1   2 , Lifei Wang  1   2 , Caihong Zheng  1 , Jun Cai  1   2
Affiliations

DSMNC: a database of somatic mutations in normal cells

Xuexia Miao et al. Nucleic Acids Res. .

Abstract

Numerous non-inherited somatic mutations, distinct from those of germ-line origin, occur in somatic cells during DNA replication per cell-division. The somatic mutations, recording the unique genetic cell-lineage 'history' of each proliferating normal cell, are important but remain to be investigated because of their ultra-low frequency hidden in the genetic background of heterogeneous cells. Luckily, the recent development of single-cell genomics biotechnologies enables the screening and collection of the somatic mutations, especial single nucleotide variations (SNVs), occurring in normal cells. Here, we established DSMNC: a database of somatic mutations in normal cells (http://dsmnc.big.ac.cn/), which provides most comprehensive catalogue of somatic SNVs in single cells from various normal tissues. In the current version, the database collected ∼0.8 million SNVs accumulated in ∼600 single normal cells (579 human cells and 39 mouse cells). The database interface supports the user-friendly capability of browsing and searching the SNVs and their annotation information. DSMNC, which serves as a timely and valuable collection of somatic mutations in individual normal cells, has made it possible to analyze the burdens and signatures of somatic mutations in various types of heterogeneous normal cells. Therefore, DSMNC will significantly improve our understanding of the characteristics of somatic mutations in normal cells.

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Figures

Figure 1.
Figure 1.
Schematically illustrates the general workflow and features of the database.
Figure 2.
Figure 2.
Screenshots of the web interfaces in DSMNC. The web interface of DSMNC comprises four main functional components: Home webpage, Browse webpage, Search webpage and Download webpage. The view of DSMNC database summary is given in the Home webpage. Users can browse the detailed genomic information on selected group of somatic SNVs in text format or visualized image format. The Search webpage allows users to retrieve the list of somatic SNVs indexed by gene symbols or chromosome regions. And somatic SNVs indexed by the accession ID for each single cell can be downloaded in the Download webpage.
Figure 3.
Figure 3.
Prospective analysis on SNV signatures in DSMNC. (A) Mutation loads in normal single cells from different types of human tissues. (B) The somatic SNV spectra in normal cells comparing with the SNP spectra. The SNP information of Human and Mouse was retrieved from the dbSNP137 and dbSNP128, respectively.
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