Soil samples (collected from El-Madina El-Monawara, Kingdom Saudi Arabia) were mixed with human saliva, incubated in media suitable for bacterial and fungal growth and filtered. Eighteen bacterial and five fungal species were isolated and identified. The bacterial and fungal filtrates as well as the isolated species were evaluated for their antimicrobial activities against some pathogenic microbes causing dermatological diseases (Staphylococcus aureus, methicillin resistant S. aureus (MRSA) and Aspergillus niger). The bacterial filtrate showed significant antagonistic effect against S. aureus and methicillin resistant S. aureus (MRSA), whereas showed non inhibitory action on the pathogenic fungus. In contrast, the fungal filtrate antagonized the growth of the pathogenic fungus (A. niger) and did not produce any inhibitory effect on the two tested pathogenic bacteria. The isolated bacterial species showed different levels of antagonistic activities against the three tested microbes. Bacillus subtilis was described as potent isolate against the three pathogens, followed by Esherichia coli. However, Bacillus megaterium strongly inhibited the growth of the pathogenic bacteria only. On the other side, all the fungal filtrates of the isolated species, except Cochliobolus lanatus showed antagonistic activity against the pathogenic fungus (A. niger). The filtrate of Fusarium oxysporum and Emericella nidulans counteracted the growth of S. aureus, whereas, the growth of MRSA was inhibited only by the filtrate of E. nidulans. From the passage way of our respected prophet, how is never tells from him self, if any person complains from awound or ulcer, the messenger of Allah (prayers and peace be upon him) put his forefinger on the ground and lift it then he says: (In the Name of God, soil of our land, with the saliva of some of us, our sick person will get well after the permission of our God) Al-Bukhari. The meaning of this Hadith that the prophet takes his saliva on the forefinger then he put it on the soil and wipe on the wound place while saying the above Hadith that is shows the Prophet's miracle, which is evidence of healing by using soil and saliva.