Myristic acid is coupled to a structural protein of polyoma virus and SV40
- PMID: 3031509
- DOI: 10.1038/326619a0
Myristic acid is coupled to a structural protein of polyoma virus and SV40
Abstract
In the lytic cycle of papova viruses, both uncoating of the viral genome after infection and assembly of functional virions take place in the cell nucleus. The mechanisms by which newly internalized virions are targeted to the nucleus and viral DNA encapsidated into particles are poorly understood. Although the major capsid protein VP1 is involved in endocytosis, and largely defines virion structure, the functions of the minor proteins VP2 and VP3 have remained obscure. Here we show that VP2 from both polyoma virus and simian virus 40 (SV40) is covalently linked to myristic acid; this is the first report of a myristylated protein in the nucleus and of a fatty acid being important in the structure of a nonenveloped virus. We consider the implications of this unusual modification on encapsidation and suggest that VP2 may be a scaffolding protein for virion assembly.
Similar articles
-
The primary sequence of the late region of polyoma virus DNA II. The expression of the late genes and comparison with DNA sequences of SV40 and BKV.Nucleic Acids Symp Ser. 1979;(6):s157-60. Nucleic Acids Symp Ser. 1979. PMID: 232759
-
SV40 large T antigen functions at two distinct steps in virion assembly.Virology. 1994 Oct;204(1):200-9. doi: 10.1006/viro.1994.1524. Virology. 1994. PMID: 8091652
-
Expression of the polyomavirus VP2 and VP3 proteins in insect cells: coexpression with the major capsid protein VP1 alters VP2/VP3 subcellular localization.Virology. 1993 May;194(1):393-8. doi: 10.1006/viro.1993.1274. Virology. 1993. PMID: 8386884
-
Viral acylproteins: greasing the wheels of assembly.Trends Microbiol. 1993 Apr;1(1):20-5. doi: 10.1016/0966-842x(93)90020-r. Trends Microbiol. 1993. PMID: 8143111 Review.
-
Activation of the abl oncogene in murine and human leukemias.Biochim Biophys Acta. 1985 Nov 12;823(1):1-17. doi: 10.1016/0304-419x(85)90012-5. Biochim Biophys Acta. 1985. PMID: 2996602 Review. No abstract available.
Cited by
-
Myristoylation of the small envelope protein of porcine reproductive and respiratory syndrome virus is non-essential for virus infectivity but promotes its growth.Virus Res. 2010 Feb;147(2):294-9. doi: 10.1016/j.virusres.2009.11.016. Epub 2009 Nov 29. Virus Res. 2010. PMID: 19951726 Free PMC article.
-
Fatty acylation of proteins.Biochim Biophys Acta. 1989 Dec 6;988(3):411-26. doi: 10.1016/0304-4157(89)90013-0. Biochim Biophys Acta. 1989. PMID: 2686758 Free PMC article. Review. No abstract available.
-
Fatty acid acylation of vaccinia virus proteins.J Virol. 1989 Oct;63(10):4285-91. doi: 10.1128/JVI.63.10.4285-4291.1989. J Virol. 1989. PMID: 2778876 Free PMC article.
-
Myristoylation and the post-translational acquisition of hydrophobicity by the membrane immunoglobulin heavy-chain polypeptide in B lymphocytes.Proc Natl Acad Sci U S A. 1987 Nov;84(21):7654-8. doi: 10.1073/pnas.84.21.7654. Proc Natl Acad Sci U S A. 1987. PMID: 3118373 Free PMC article.
-
Minor capsid proteins of simian virus 40 are dispensable for nucleocapsid assembly and cell entry but are required for nuclear entry of the viral genome.J Virol. 2007 Apr;81(8):3778-85. doi: 10.1128/JVI.02664-06. Epub 2007 Jan 31. J Virol. 2007. PMID: 17267496 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
