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. 1987 Feb;61(2):436-45.
doi: 10.1128/JVI.61.2.436-445.1987.

trans-dominant defective mutants of simian virus 40 T antigen

J M Farber, K W Peden, D Nathans

trans-dominant defective mutants of simian virus 40 T antigen

J M Farber et al. J Virol. 1987 Feb.

Abstract

We constructed a collection of linker insertion mutants in the simian virus 40 (SV40) genome and studied several of these with changes limited to a part of the large T antigen gene corresponding to an amino acid sequence shared with other ATPases. Two of these mutants were found to have a novel phenotype in that they could not be complemented for plaque formation by a late-region deletion mutant. These two mutants, in contrast to other mutants in this region, were able to transform rat cells in culture at a frequency close to that of the wild-type gene. The noncomplementing mutants were found to be potent inhibitors of SV40 DNA replication despite the presence of wild-type T antigen in the transfected cells. This inhibition was shown to be the result of the introduced mutations in the large T antigen gene. We conclude that the large T antigens of the noncomplementing mutants can act as inhibitors of SV40 DNA replication.

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References

    1. EMBO J. 1985 Nov;4(11):2933-9 - PubMed
    1. Mol Cell Biol. 1982 Dec;2(12):1463-71 - PubMed
    1. Virology. 1964 May;23(1):117-9 - PubMed
    1. Nature. 1968 Mar 23;217(5134):1110-4 - PubMed
    1. Virology. 1971 Jul;45(1):163-71 - PubMed

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