Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Oct;205(1):127-33.
doi: 10.1007/BF02428042.

The carboxy-terminal region of haemolysin 2001 is required for secretion of the toxin from Escherichia coli

L Gray, N Mackman, J M Nicaud, I B Holland

The carboxy-terminal region of haemolysin 2001 is required for secretion of the toxin from Escherichia coli

L Gray et al. Mol Gen Genet. 1986 Oct.

Abstract

As a first step in the detailed analysis of the mechanism of secretion of haemolysin, we sought to identify sequences or domains within haemolysin A (HlyA) that are essential for its secretion. For this purpose we examined the properties of a deletion and Tn5 insertions into the region of the HlyA gene encoding the C-terminal part of the protein, since both of these are relatively simple to generate. We showed that removal of 27 amino acids from the C-terminus of HlyA is sufficient to inhibit secretion drastically, although the residual polypeptide is still haemolytically active. Cellular fractionation studies showed that haemolytic activity does not accumulate in large amounts within the periplasmic space during normal secretion. More significantly, activity does not appear to accumulate within this compartment when the export functions hlyB and hlyD are removed. These results are consistent with a mechanism in which interaction of the C-terminus of HlyA with the secretion machinery, located in the inner membrane, is followed by direct transfer of haemolysin to the medium.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Infect Immun. 1986 Apr;52(1):63-9 - PubMed
    1. Microbiol Rev. 1983 Sep;47(3):313-44 - PubMed
    1. J Biol Chem. 1966 Jul 10;241(13):3055-62 - PubMed
    1. FEBS Lett. 1985 Aug 5;187(2):339-44 - PubMed
    1. J Mol Biol. 1982 May 5;157(1):105-32 - PubMed

Publication types

LinkOut - more resources