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. 1987 Jan;61(1):119-24.
doi: 10.1128/JVI.61.1.119-124.1987.

Genomic localization, sequence analysis, and transcription of the putative human cytomegalovirus DNA polymerase gene

R Heilbronn, G Jahn, A Bürkle, U K Freese, B Fleckenstein, H zur Hausen

Genomic localization, sequence analysis, and transcription of the putative human cytomegalovirus DNA polymerase gene

R Heilbronn et al. J Virol. 1987 Jan.

Abstract

The human cytomegalovirus (HCMV)-induced DNA polymerase has been well characterized biochemically and functionally, but its genomic location has not yet been assigned. To identify the coding sequence, cross-hybridization with the herpes simplex virus type 1 (HSV-1) polymerase gene was used, as suggested by the close similarity of the herpes group virus-induced DNA polymerases to the HCMV DNA polymerase. A cosmid and plasmid library of the entire HCMV genome was screened with the BamHI Q fragment of HSV-1 at different stringency conditions. One PstI-HincII restriction fragment of 850 base pairs mapping within the EcoRI M fragment of HCMV cross-hybridized at Tm - 25 degrees C. Sequence analysis revealed one open reading frame spanning the entire sequence. The amino acid sequence showed a highly conserved domain of 133 amino acids shared with the HSV and putative Epstein-Barr virus polymerase sequences. This domain maps within the C-terminal part of the HSV polymerase gene, which has been suggested to contain part of the catalytic center of the enzyme. Transcription analysis revealed one 5.4-kilobase early transcript in the sense orientation with respect to the open reading frame identified. This transcript appears to code for the 140-kilodalton HCMV polymerase protein.

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References

    1. J Virol. 1975 Aug;16(2):298-310 - PubMed
    1. J Virol. 1987 Jan;61(1):125-33 - PubMed
    1. Arch Virol. 1976;52(4):333-9 - PubMed
    1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
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