Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018:1861:47-53.
doi: 10.1007/978-1-4939-8766-5_4.

Manipulation of Xist Imprinting in Mouse Preimplantation Embryos

Atsushi Fukuda  1   2 , Akihiro Umezawa  3 , Hidenori Akutsu  3
Affiliations

Manipulation of Xist Imprinting in Mouse Preimplantation Embryos

Atsushi Fukuda et al. Methods Mol Biol. 2018.

Abstract

X-chromosome inactivation (XCI) is established in preimplantation embryos in mice. Prior to the establishment of XCI, expression of the long noncoding RNA (lncRNA) Xist is essential. Xist expression in mouse preimplantation embryos is imprinted, and paternal Xist is predominantly expressed. Due to the manner of imprinting, maternal Xist is always silenced. However, the nature of imprinting to repress maternal Xist is variable. For example, parthenogenetic embryos that are composed of two maternal genomes exhibit maternal Xist derepression during the preimplantation phases. Maintenance of Xist imprinting to repress maternal Xist depends on the chromatin condensation states and the dosage of Rnf12, an essential Xist activator. Therefore, alterations of chromatin states and Rnf12 expression levels lead to maternal Xist derepression. In this chapter, we describe the method for derepressing maternal Xist by various approaches, such as mRNA injection, small molecule treatment, and nuclear transfer.

Keywords: Chromatin condensation; Imprinted XCI; Maternal Xist.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

Publication types

LinkOut - more resources