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. 2018 Sep;4(3):300-304.
doi: 10.1016/j.aninu.2018.06.003. Epub 2018 Jul 10.

Enzyme activity and phosphate uptake in the small intestine of Sprague Dawley rats improved by supplementation of infant formula with prebiotics

Shuiyue Zhou  1 , Yuanxin Hang  2 , Jianwu Wang  2 , Rejun Fang  1
Affiliations

Enzyme activity and phosphate uptake in the small intestine of Sprague Dawley rats improved by supplementation of infant formula with prebiotics

Shuiyue Zhou et al. Anim Nutr. 2018 Sep.

Abstract

This study was to identify the effects of prebiotics supplemented in infant formula on enzyme activity and phosphate uptake in the small intestine of Sprague Dawley (SD) rats. Forty-eight healthy SD rats at 15 days old (a week before weaning) with similar weight were randomly divided into 3 groups: A (control group), B, C, with 16 rats per group. Rats in groups A, B, C were fed a standard infant formula, the standard infant formula supplemented with oligosaccharides, and the standard infant formula supplemented with polysaccharides, respectively. The feeding test was conducted for 28 d. Compared with group A, the results showed the following: 1) the activities of sucrose and lactase in the small intestine were significantly increased in SD rats of group C (P < 0.05); 2) the relative expressions of lactase gene in the anterior and posterior segments of the small intestine were significantly increased by 1.68 and 2.26 in SD rats of group C (P < 0.05), and the relative expression of Mgam gene in the posterior segment of the small intestine was significantly increased by 0.99 in SD rats of group C (P < 0.05); 3) the relative expressions of Na/Pi-IIb gene in the anterior and posterior segments of the small intestine were significantly increased by 1.85 and 2.28 in SD rats of group C (P < 0.05). These results indicate that the supplementation of infant formula with prebiotics can promote enzyme activity in the small intestine by increasing the relative expression of enzyme gene or by decreasing intestinal injury, and can increase the relative expression of Na/Pi-IIb gene. The effect of polysaccharides is better than that of oligosaccharides.

Keywords: Infant formula; Intestinal enzyme activities; Phosphate absorption; Prebiotics; Ussing chamber.

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Figures

Fig. 1
Fig. 1
Lactase (A) and sucrase (B) activities in the small intestine of Sprague Dawley (SD) rats in different groups. * means significant difference among 3 groups (P < 0.05).
Fig. 2
Fig. 2
Relative expressions of enzyme genes, Lactase (A) and Mgam (B), in the small intestine of Sprague Dawley (SD) rats in different groups. * means significant difference among 3 groups (P < 0.05).
Fig. 3
Fig. 3
Phosphate in vitro absorption rate in the small intestine of Sprague Dawley (SD) rats in different groups.
Fig. 4
Fig. 4
The relative expression of Na/Pi-IIb gene in the small intestine of Sprague Dawley (SD) rats in different groups. * means significant difference among 3 groups (P < 0.05).
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