The myeloproliferative sarcoma virus retains transforming functions after introduction of a dominant selectable marker gene
- PMID: 3014049
- DOI: 10.1099/0022-1317-67-7-1361
The myeloproliferative sarcoma virus retains transforming functions after introduction of a dominant selectable marker gene
Abstract
The dominant neomycin resistance gene (neoR) was introduced into the genome of the myeloproliferative sarcoma virus (MPSV), a replication-defective retrovirus carrying the mos oncogene. The resulting selectable neoR-MPSV virus did not lose its acute transforming property, unlike the results of attempts by other groups to insert marker genes into oncogenic viruses. NeoR-MPSV DNA was used to generate infectious virus by transfection followed by rescue with Friend or Moloney murine leukaemia virus. Infection of fibroblasts with this virus resulted in morphologically transformed cells which were resistant to the neomycin analogue G418. Segregation of the two functions (transformation and G418 resistance) was not observed in more than 500 independent viral transfers to fibroblasts. Furthermore, neoR-MPSV retained the leukaemogenesis-inducing properties of the wild-type virus. Myeloproliferation and G418-resistance transfer did not segregate after passage in mice.
Similar articles
-
Viral transfer, transcription, and rescue of a selectable myeloproliferative sarcoma virus in embryonal cell lines: expression of the mos oncogene.Mol Cell Biol. 1986 Jan;6(1):286-93. doi: 10.1128/mcb.6.1.286-293.1986. Mol Cell Biol. 1986. PMID: 3023829 Free PMC article.
-
Stable expression of a selectable myeloproliferative sarcoma virus in murine T lymphocyte and monocyte cell lines.Immunobiology. 1987 May;174(3):313-25. doi: 10.1016/S0171-2985(87)80006-2. Immunobiology. 1987. PMID: 3040583
-
Retroviral mutants efficiently expressed in embryonal carcinoma cells.Proc Natl Acad Sci U S A. 1986 May;83(10):3292-6. doi: 10.1073/pnas.83.10.3292. Proc Natl Acad Sci U S A. 1986. PMID: 3010288 Free PMC article.
-
The ts110 Moloney mouse sarcoma virus system: gag-mos gene products and cellular transformation.J Gen Virol. 1985 Sep;66 ( Pt 9):1845-53. doi: 10.1099/0022-1317-66-9-1845. J Gen Virol. 1985. PMID: 2993477 Review. No abstract available.
-
Murine leukemia viruses with recombinant env genes: a discussion of their role in leukemogenesis.Curr Top Microbiol Immunol. 1983;103:75-108. doi: 10.1007/978-3-642-68943-7_4. Curr Top Microbiol Immunol. 1983. PMID: 6303710 Review. No abstract available.
Cited by
-
Autocrine stimulation after transfer of the granulocyte/macrophage colony-stimulating factor gene and autonomous growth are distinct but interdependent steps in the oncogenic pathway.Proc Natl Acad Sci U S A. 1987 Dec;84(23):8458-62. doi: 10.1073/pnas.84.23.8458. Proc Natl Acad Sci U S A. 1987. PMID: 3317408 Free PMC article.
-
Murine gamma interferon inhibits v-mos-induced fibroblast transformation via down regulation of retroviral gene expression.J Virol. 1987 Aug;61(8):2567-72. doi: 10.1128/JVI.61.8.2567-2572.1987. J Virol. 1987. PMID: 3037117 Free PMC article.
-
Effects of an internal transcription unit and its orientation on retrovirus titre and expression.Cytotechnology. 1997 May;24(1):31-8. doi: 10.1023/A:1007956013403. Cytotechnology. 1997. PMID: 22358594 Free PMC article.
-
Gamma interferon regulates long terminal repeat-controlled oncogene expression in transformed mouse fibroblasts at the level of mRNA transcription.J Virol. 1988 Feb;62(2):619-21. doi: 10.1128/JVI.62.2.619-621.1988. J Virol. 1988. PMID: 3121865 Free PMC article.
-
Short-term treatment with gamma interferon induces stable reversion of ras-transformed mouse fibroblasts.J Virol. 1991 Nov;65(11):6307-11. doi: 10.1128/JVI.65.11.6307-6311.1991. J Virol. 1991. PMID: 1717721 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
