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. 2018 Nov 1;526(16):2631-2646.
doi: 10.1002/cne.24521. Epub 2018 Oct 23.

Heterogeneous distribution of doublecortin-expressing cells surrounding the rostral migratory stream in the juvenile mouse

Yuka Aoyagi  1   2 , Terumasa Hibi  1   2 , Yoshitaka Kimori  3 , Masato Sawada  4 , Ryosuke Kawakami  1   2 , Kazunobu Sawamoto  4   5 , Tomomi Nemoto  1   2
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Free article

Heterogeneous distribution of doublecortin-expressing cells surrounding the rostral migratory stream in the juvenile mouse

Yuka Aoyagi et al. J Comp Neurol. .
Free article

Abstract

In the postnatal mammalian brain, neural stem cells of the ventricular-subventricular zone continue to generate doublecortin (Dcx)-expressing immature neurons. Throughout life, these immature neurons migrate to the olfactory bulb through the rostral migratory stream (RMS). In this study, we investigated the distribution of these putative immature neurons using enhanced green fluorescent protein (EGFP) expression in the area surrounding the RMS of the juvenile Dcx-EGFP mice. Through the combined use of an optical clearing reagent (a 2,2'-thiodiethanol solution) and two-photon microscopy, we visualized three-dimensionally the EGFP-positive cells in the entire RMS and its surroundings. The resulting wide-field and high-definition images along with computational image processing methods developed in this study were used to comprehensively determine the position of the EGFP-positive cells. Our findings revealed that the EGFP-positive cells were heterogeneously distributed in the area surrounding the RMS. In addition, the orientation patterns of the leading process of these cells, which displayed the morphology of migrating immature neurons, differed depending on their location. These novel results provide highly precise morphological information for immature neurons and suggest that a portion of immature neurons may be detached from the RMS and migrate in various directions.

Keywords: RRID: AB_141607; RRID: AB_141633; RRID: AB_141637; RRID: AB_141708; RRID: AB_2298772; RRID: AB_477010; RRID: AB_561007; RRID: AB_591819; RRID: AB_664697; computer-assisted image processing; multiphoton fluorescence microscopy; neurogenesis, neural stem cells; three-dimensional imaging.

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