Development and application of bioluminescence imaging for the influenza A virus

doi:10.21037/jtd.2018.02.35

Review

. 2018 Jul;10(Suppl 19):S2230-S2237.

doi: 10.21037/jtd.2018.02.35.

Development and application of bioluminescence imaging for the influenza A virus

Weiqi Pan¹, Ji Dong¹, Peihai Chen^{2

3}, Beiwu Zhang², Zhixia Li², Ling Chen^{1

2}

Affiliations

¹ State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510182, China.
² Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
³ Institute of Health Sciences, Anhui University, Hefei 230601, China.

PMID: 30116602
PMCID: PMC6072922
DOI: 10.21037/jtd.2018.02.35

Review

Development and application of bioluminescence imaging for the influenza A virus

Weiqi Pan et al. J Thorac Dis. 2018 Jul.

. 2018 Jul;10(Suppl 19):S2230-S2237.

doi: 10.21037/jtd.2018.02.35.

Authors

Weiqi Pan¹, Ji Dong¹, Peihai Chen^{2

3}, Beiwu Zhang², Zhixia Li², Ling Chen^{1

2}

Affiliations

¹ State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510182, China.
² Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
³ Institute of Health Sciences, Anhui University, Hefei 230601, China.

PMID: 30116602
PMCID: PMC6072922
DOI: 10.21037/jtd.2018.02.35

Abstract

Influenza A viruses (IAVs) cause seasonal epidemics and intermittent pandemics which threaten human health. Conventional assays cannot meet the demands for rapid and sensitive detection of viral spread and pathogenesis in real time cannot be used for high-throughput screens of novel antivirals. Bioluminescence imaging (BLI) has emerged as a powerful tool in the study of infectious diseases in animal models. The advent of influenza reverse genetics has enabled the incorporation of bioluminescent reporter proteins into replication-competent IAVs. This review briefly describes the current development and applications of bioluminescence in the study of viral infections and antiviral therapeutics for IAVs. BLI is expected to substantially accelerate the basic and applied research of IAV both in vitro and in vivo.

Keywords: Bioluminescence imaging (BLI); influenza A virus (IAV); luciferase; replication-competent.

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Conflict of interest statement

Conflicts of Interest: The authors have no conflicts of interest to declare.

Figures

**Figure 1**
Bioluminescence imaging of mice infected with IAV-Luc virus. Balb/c mice (6–8 weeks old) were infected with 106 pfu of IAV-Luc. Mice were imaged by IVIS200 at 24 h intervals from 24 to 144 h post infection after injection of coelenterazine. IVA, influenza A virus; ROI, region of interest.

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[1] Palese P, Shaw ML. Orthomyxoviridae: The viruses and their replication. In: Knipe DM, Howley PM, Griffin DE, et al. editors. Fields Virology. Philadelphia: Lippincott Williams and Wilkins, 2007.

[2] Palese P, Shaw ML. Orthomyxoviridae: The viruses and their replication. In: Knipe DM, Howley PM, Griffin DE, et al. editors. Fields Virology. Philadelphia: Lippincott Williams and Wilkins, 2007.

[3] Tong S, Zhu X, Li Y, et al. New world bats harbor diverse influenza A viruses. PLoS Pathog 2013. 9:e1003657. 10.1371/journal.ppat.1003657 - DOI - PMC - PubMed

[4] Tong S, Zhu X, Li Y, et al. New world bats harbor diverse influenza A viruses. PLoS Pathog 2013. 9:e1003657. 10.1371/journal.ppat.1003657 - DOI - PMC - PubMed

[5] Tong S, Li Y, Rivailler P, et al. A distinct lineage of influenza A virus form bats. Proc Natl Acad Sci U S A 2012;109:4269-74. 10.1073/pnas.1116200109 - DOI - PMC - PubMed

[6] Tong S, Li Y, Rivailler P, et al. A distinct lineage of influenza A virus form bats. Proc Natl Acad Sci U S A 2012;109:4269-74. 10.1073/pnas.1116200109 - DOI - PMC - PubMed

[7] Horimoto T, Kawaoka Y. Influenza: lessons from past pandemics, warnings from current incidents. Nat Rev Microbiol 2005;3:591-600. 10.1038/nrmicro1208 - DOI - PubMed

[8] Horimoto T, Kawaoka Y. Influenza: lessons from past pandemics, warnings from current incidents. Nat Rev Microbiol 2005;3:591-600. 10.1038/nrmicro1208 - DOI - PubMed

[9] Clem A, Galwankar S. Seasonal influenza: waiting for the next pandemic. J Glob Infect Dis 2009;1:51-6. 10.4103/0974-777X.52983 - DOI - PMC - PubMed

[10] Clem A, Galwankar S. Seasonal influenza: waiting for the next pandemic. J Glob Infect Dis 2009;1:51-6. 10.4103/0974-777X.52983 - DOI - PMC - PubMed

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Development and application of bioluminescence imaging for the influenza A virus

Affiliations

Development and application of bioluminescence imaging for the influenza A virus

Authors

Affiliations

Abstract

Conflict of interest statement

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