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. 2018 Jul;63(1):50-57.
doi: 10.3164/jcbn.17-96. Epub 2018 Apr 3.

Effects of vitamin C and E on toxic action of alcohol on partial hepatectomy-induced liver regeneration in rats

Yurika Okamura  1 , Akira Omori  2 , Norihiko Asada  3 , Akifumi Ono  4
Affiliations

Effects of vitamin C and E on toxic action of alcohol on partial hepatectomy-induced liver regeneration in rats

Yurika Okamura et al. J Clin Biochem Nutr. 2018 Jul.

Abstract

The purpose of this study was to investigate the influence of vitamins C and E on the toxic action of alcohol in rat liver regeneration. Male Sprague-Dawley rats subjected to 70% partial hepatectomy were divided into five groups (Groups 1 to 5). Rats in Groups 2 to 5 were only provided alcohol for drinking. Additionally, vitamin C, vitamin E, and vitamin C in combination with vitamin E were administered to Groups 3, 4, and 5, respectively. Alcohol inhibits liver regeneration, resulting in an increase in free radicals produced by alcohol metabolism and thus causing cellular damage and altering liver function. During liver regeneration, vitamins C and E significantly ameliorated liver injury from alcohol administration by reducing hepatic lipid peroxidation. Vitamins C and E protect against liver injury and dysfunction, attenuate lipid peroxidation, and thus may be more effective in combination than either vitamin alone against alcohol-mediated toxic effects during liver regeneration.

Keywords: alcohol; antioxidant; liver regeneration; oxidative stress; partial hepatectomy.

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Conflict of interest statement

We have not received any financial support or other benefits from commercial sources for the work reported in the manuscript. None of the authors have financial interests that could create a potential conflict of interest or the appearance of a conflict of interest with regard to this work.

Figures

Fig. 1
Fig. 1
The effect of alcohol and vitamin administration on body weight before and after partial hepatectomy. ◯, Partial hepatectomy + Control (PH + C); ●, Partial hepatectomy + Alcohol (PH + A); △, Partial hepatectomy + Alcohol + Vitamin C (PH + A + Vit.C); ■, Partial hepatectomy + Alcohol + Vitamin E (PH + A + Vit.E); □, Partial hepatectomy + Alcohol + Vitamin C and E (PH + A + Vit.C and E) groups. Data represent means ± SD. *p<0.05.
Fig. 2
Fig. 2
Malondialdehyde (MDA) levels in rat serum 1 week and 5 weeks after partial hepatectomy and daily ingestion of alcohol and administration of vitamins C and/or E. Partial hepatectomy + Control (PH + C), Partial hepatectomy + Alcohol (PH + A), Partial hepatectomy + Alcohol + Vitamin C (PH + A + Vit.C; VC), Partial hepatectomy + Alcohol + Vitamin E (PH + A + Vit.E; VE), Partial hepatectomy + Alcohol + Vitamin C and E (PH + A + Vit.C and E; VC,E) groups. All values are expressed as means ± SD. Different superscript letters indicate significant differences for each group (p<0.05).
Fig. 3
Fig. 3
Serum alanine aminotransferase (ALT) levels in rats 1 week and 5 weeks after partial hepatectomy and daily ingestion of alcohol and administration of vitamins C and/or E. Partial hepatectomy + Control (PH + C), Partial hepatectomy + Alcohol (PH + A), Partial hepatectomy + Alcohol + Vitamin C (PH + A + Vit.C; VC), Partial hepatectomy + Alcohol + Vitamin E (PH + A + Vit.E; VE), Partial hepatectomy + Alcohol + Vitamin C and E (PH + A + Vit.C and E; VC,E) groups. All values are expressed as means ± SD. Different superscript letters indicate significant differences for each group (p<0.05).
Fig. 4
Fig. 4
Serum aspartate aminotransferase (AST) levels in rats 1 week and 5 weeks after partial hepatectomy and daily ingestion of alcohol and administration of vitamins C and/or E. Partial hepatectomy + Control (PH + C), Partial hepatectomy + Alcohol (PH + A), Partial hepatectomy + Alcohol + Vitamin C (PH + A + Vit.C; VC), Partial hepatectomy + Alcohol + Vitamin E (PH + A + Vit.E; VE), Partial hepatectomy + Alcohol + Vitamin C and E (PH + A + Vit.C and E; VC,E) groups. All values are expressed as means ± SD.
Fig. 5
Fig. 5
Albumin levels in serum from rats 1 week and 5 weeks after partial hepatectomy and daily ingestion of alcohol and administration of vitamins C and/or E. Partial hepatectomy + Control (PH + C), Partial hepatectomy + Alcohol (PH + A), Partial hepatectomy + Alcohol + Vitamin C (PH + A + Vit.C; VC), Partial hepatectomy + Alcohol + Vitamin E (PH + A + Vit.E; VE), Partial hepatectomy + Alcohol + Vitamin C and E (PH + A + Vit.C and E; VC,E) groups. All values are expressed as means ± SD. Different superscript letters indicate significant differences for each group (p<0.05).
Fig. 6
Fig. 6
Total bilirubin (T-bilirubin) levels in serum from rats 1 week and 5 weeks after partial hepatectomy and daily ingestion of alcohol and administration of vitamins C and/or E. Partial hepatectomy + Control (PH + C), Partial hepatectomy + Alcohol (PH + A), Partial hepatectomy + Alcohol + Vitamin C (PH + A + Vit.C; VC), Partial hepatectomy + Alcohol + Vitamin E (PH + A + Vit.E; VE), Partial hepatectomy + Alcohol + Vitamin C and E (PH + A + Vit.C and E; VC,E) groups. All values are expressed as means ± SD.
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