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. 1986 Apr;166(1):100-4.
doi: 10.1128/jb.166.1.100-104.1986.

F plasmid ccd mechanism in Escherichia coli

S Hiraga, A Jaffé, T Ogura, H Mori, H Takahashi

F plasmid ccd mechanism in Escherichia coli

S Hiraga et al. J Bacteriol. 1986 Apr.

Abstract

The ccd mechanism specified by the ccdA and ccdB genes of the mini-F plasmid determines fate of plasmid-free segregants in Escherichia coli (Jaffé et al., J. Bacteriol. 163:841-849, 1985). The killing function in plasmid-free segregants by the ccd mechanism did not affect cell growth of coexisting cells in the same culture. Elongated cells and anucleate cells caused by the ccd mechanism were clearly detected by flow cytometry in cultures of bacterial strains harboring Ccd+ Sop- mini-F plasmids defective in partitioning. This indicates that the defect in correct partitioning of plasmid DNA molecules into daughter cells also induces the ccd mechanism to operate.

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