Regulation of global translation during the cell cycle
- PMID: 30072440
- DOI: 10.1242/jcs.220327
Regulation of global translation during the cell cycle
Abstract
It is generally accepted that global translation varies during the cell cycle and is low during mitosis. However, addressing this issue is challenging because it involves cell synchronization, which evokes stress responses that, in turn, affect translation rates. Here, we have used two approaches to measure global translation rates in different cell-cycle phases. First, synchrony in different cell-cycle phases was obtained involving the same stress, by using temperature-sensitive mutants. Second, translation and DNA content were measured by flow cytometry in exponentially growing, single cells. We found no major variation in global translation rates through the cell cycle in either fission yeast or mammalian cells. We also measured phosphorylation of eukaryotic initiation factor-2α, an event that is thought to downregulate global translation in mitosis. In contrast with the prevailing view, eIF2α phosphorylation correlated poorly with downregulation of global translation and ectopically induced eIF2α phosphorylation inhibited global translation only at high levels.
Keywords: Cell cycle; EIF2α phosphorylation; Translation.
© 2018. Published by The Company of Biologists Ltd.
Conflict of interest statement
Competing interestsThe authors declare no competing or financial interests.
Similar articles
-
Stress-induced inhibition of translation independently of eIF2α phosphorylation.J Cell Sci. 2015 Dec 1;128(23):4420-7. doi: 10.1242/jcs.176545. Epub 2015 Oct 22. J Cell Sci. 2015. PMID: 26493332 Free PMC article.
-
Stress-activated protein kinase pathway functions to support protein synthesis and translational adaptation in response to environmental stress in fission yeast.Eukaryot Cell. 2005 Nov;4(11):1785-93. doi: 10.1128/EC.4.11.1785-1793.2005. Eukaryot Cell. 2005. PMID: 16278445 Free PMC article.
-
Fission yeast mitogen-activated protein kinase Sty1 interacts with translation factors.Eukaryot Cell. 2008 Feb;7(2):328-38. doi: 10.1128/EC.00358-07. Epub 2007 Dec 7. Eukaryot Cell. 2008. PMID: 18065650 Free PMC article.
-
Controlling cell cycle progress in the fission yeast Schizosaccharomyces pombe.Curr Opin Genet Dev. 1991 Oct;1(3):307-12. doi: 10.1016/s0959-437x(05)80292-8. Curr Opin Genet Dev. 1991. PMID: 1840886 Review.
-
eIF2α phosphorylation and the regulation of translation.Curr Genet. 2020 Apr;66(2):293-297. doi: 10.1007/s00294-019-01026-1. Epub 2019 Sep 4. Curr Genet. 2020. PMID: 31485739 Review.
Cited by
-
Regulating the regulator: a survey of mechanisms from transcription to translation controlling expression of mammalian cell cycle kinase Aurora A.Open Biol. 2022 Sep;12(9):220134. doi: 10.1098/rsob.220134. Epub 2022 Sep 7. Open Biol. 2022. PMID: 36067794 Free PMC article. Review.
-
TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment.iScience. 2023 Mar 31;26(4):106543. doi: 10.1016/j.isci.2023.106543. eCollection 2023 Apr 21. iScience. 2023. PMID: 37123244 Free PMC article.
-
miR-31-mediated local translation at the mitotic spindle is important for early development.Development. 2024 Sep 1;151(17):dev202619. doi: 10.1242/dev.202619. Epub 2024 Sep 5. Development. 2024. PMID: 39250531
-
The cell cycle and cell size influence the rates of global cellular translation and transcription in fission yeast.EMBO J. 2023 May 2;42(9):e113333. doi: 10.15252/embj.2022113333. Epub 2023 Mar 23. EMBO J. 2023. PMID: 36951016 Free PMC article.
-
Codon usage and protein length-dependent feedback from translation elongation regulates translation initiation and elongation speed.Nucleic Acids Res. 2021 Sep 20;49(16):9404-9423. doi: 10.1093/nar/gkab729. Nucleic Acids Res. 2021. PMID: 34417614 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
