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. 1986 Feb;83(3):571-5.
doi: 10.1073/pnas.83.3.571.

A poliovirus temperature-sensitive RNA synthesis mutant located in a noncoding region of the genome

P Sarnow, H D Bernstein, D Baltimore

A poliovirus temperature-sensitive RNA synthesis mutant located in a noncoding region of the genome

P Sarnow et al. Proc Natl Acad Sci U S A. 1986 Feb.

Abstract

We have constructed an 8-base-pair insertion mutation in the 3' noncoding region of an infectious poliovirus cDNA clone that gives rise to a temperature-sensitive RNA synthesis mutant upon transfection into mammalian cells. The mutated cDNA was used to establish a cell line that releases the mutant poliovirus in a temperature-dependent fashion, representing a unique persistent viral infection. A poliovirus mutant mapping in the noncapsid region of the viral genome can be complemented in this cell line, implying that the cell line expresses viral proteins at the nonpermissive temperature.

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