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. 2018 Jul 1;25(Pt 4):1249-1260.
doi: 10.1107/S1600577518007166. Epub 2018 Jun 27.

ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF

Andrew A McCarthy  1 , Ray Barrett  2 , Antonia Beteva  2 , Hugo Caserotto  2 , Fabien Dobias  2 , Franck Felisaz  1 , Thierry Giraud  2 , Matias Guijarro  2 , Robert Janocha  1 , Akim Khadrouche  1 , Mario Lentini  2 , Gordon A Leonard  2 , Marcos Lopez Marrero  1 , Stephanie Malbet-Monaco  2 , Sean McSweeney  2 , Didier Nurizzo  2 , Gergely Papp  1 , Christopher Rossi  1 , Jeremy Sinoir  1 , Clement Sorez  1 , John Surr  2 , Olof Svensson  2 , Ulrich Zander  1 , Florent Cipriani  1 , Pascal Theveneau  2 , Christoph Mueller-Dieckmann  2
Affiliations

ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF

Andrew A McCarthy et al. J Synchrotron Radiat. .

Abstract

ID30B is an undulator-based high-intensity, energy-tuneable (6.0-20 keV) and variable-focus (20-200 µm in diameter) macromolecular crystallography (MX) beamline at the ESRF. It was the last of the ESRF Structural Biology Group's beamlines to be constructed and commissioned as part of the ESRF's Phase I Upgrade Program and has been in user operation since June 2015. Both a modified microdiffractometer (MD2S) incorporating an in situ plate screening capability and a new flexible sample changer (the FlexHCD) were specifically developed for ID30B. Here, the authors provide the current beamline characteristics and detail how different types of MX experiments can be performed on ID30B (http://www.esrf.eu/id30b).

Keywords: FlexHCD; ID30B; MD2S; REX rapid nozzle changer; SPINEplus gripper; automation; experimental phasing.

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Figures

Figure 1
Figure 1
General overview of the ID30 complex (top) and schematic layout of ID30B (bottom). The mean distance of the optical and experimental hutches (top), and the distance of the major components (bottom) in relation to their distance from the source are also shown.
Figure 2
Figure 2
Photograph of the ID30B experimental hutch highlighting the MD2S diffractometer, REX rapid nozzle exchanger and FlexHCD sample changer. The beam path is highlighted as a red arrow.
Figure 3
Figure 3
Photon flux (photons s−1) at the sample position as a function of photon energy and beam-defining aperture used at 200 mA storage ring curent.
Figure 4
Figure 4
The MD2S on ID30B in (a) Mini-kappa (MK3) and (b) in situ plate holder goniometer head configuration.
Figure 5
Figure 5
In situ data collection on ID30B as implemented in MxCuBE.
Figure 6
Figure 6
Three types of FlexHCD grippers are currently available on ID30B: (a) a modified IRELEC flipping gripper; EMBL-designed single (b) and double (c) SPINEplus grippers. The HCD was modified to allow storage of samples in (d) SC3 or (e) Uni-Puck storage formats. Orientation and positioning aids help facilitate puck loading by users. ProxiSense puck detection sensors are used for puck type recognition, and to ensure the pucks are correctly positioned and are not inadvertently removed during sample loading.
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