CRISPR/Cas9 in plants: at play in the genome and at work for crop improvement
- PMID: 29912293
- DOI: 10.1093/bfgp/ely016
CRISPR/Cas9 in plants: at play in the genome and at work for crop improvement
Abstract
The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system uses single-guide RNAs for genome editing, making it a simple, robust, powerful tool for targeted gene mutagenesis, knockout and knock-in/replacement, as well as transcriptional regulation. Here, we review the working principles, components and potential modifications of CRISPR/Cas9 for efficient single and multiplex gene editing in plants. We also describe recent work that has used CRISPR/Cas9 to improve economically important traits in crop plants. Although the apparent ease of CRISPR/Cas9-mediated editing may make it appear as though scientists are merely playing with plant genomes, the combined power of CRISPR/Cas9 has enabled vital research to be completed in the battle toward optimization and adaptation of crop species, permitting crucial advances to be achieved in crop improvement.
Similar articles
-
CRISPR/Cas9-Based Genome Editing in Plants.Prog Mol Biol Transl Sci. 2017;149:133-150. doi: 10.1016/bs.pmbts.2017.03.008. Epub 2017 May 12. Prog Mol Biol Transl Sci. 2017. PMID: 28712494 Review.
-
Induced mutation and epigenetics modification in plants for crop improvement by targeting CRISPR/Cas9 technology.J Cell Physiol. 2018 Jun;233(6):4578-4594. doi: 10.1002/jcp.26299. Epub 2018 Jan 4. J Cell Physiol. 2018. PMID: 29194606 Review.
-
The CRISPR/Cas9 system and its applications in crop genome editing.Crit Rev Biotechnol. 2019 May;39(3):321-336. doi: 10.1080/07388551.2018.1554621. Epub 2019 Jan 15. Crit Rev Biotechnol. 2019. PMID: 30646772 Review.
-
CRISPR/Cas: A powerful tool for gene function study and crop improvement.J Adv Res. 2020 Oct 21;29:207-221. doi: 10.1016/j.jare.2020.10.003. eCollection 2021 Mar. J Adv Res. 2020. PMID: 33842017 Free PMC article. Review.
-
CRISPR-Cas9 based plant genome editing: Significance, opportunities and recent advances.Plant Physiol Biochem. 2018 Oct;131:2-11. doi: 10.1016/j.plaphy.2017.10.024. Epub 2017 Oct 27. Plant Physiol Biochem. 2018. PMID: 29103811 Review.
Cited by
-
CRISPR-Cas technology secures sustainability through its applications: a review in green biotechnology.3 Biotech. 2023 Nov;13(11):383. doi: 10.1007/s13205-023-03786-7. Epub 2023 Oct 31. 3 Biotech. 2023. PMID: 37920190 Free PMC article. Review.
-
Impact of Climate Change on Crops Adaptation and Strategies to Tackle Its Outcome: A Review.Plants (Basel). 2019 Jan 30;8(2):34. doi: 10.3390/plants8020034. Plants (Basel). 2019. PMID: 30704089 Free PMC article. Review.
-
CRISPR/Cas9 genome editing in wheat: enhancing quality and productivity for global food security-a review.Funct Integr Genomics. 2023 Aug 4;23(3):265. doi: 10.1007/s10142-023-01190-1. Funct Integr Genomics. 2023. PMID: 37541970 Review.
-
Assessment of Glyphosate Impact on the Agrofood Ecosystem.Plants (Basel). 2021 Feb 20;10(2):405. doi: 10.3390/plants10020405. Plants (Basel). 2021. PMID: 33672572 Free PMC article. Review.
-
CRISPR/Cas9 in Planta Hairy Root Transformation: A Powerful Platform for Functional Analysis of Root Traits in Soybean.Plants (Basel). 2022 Apr 12;11(8):1044. doi: 10.3390/plants11081044. Plants (Basel). 2022. PMID: 35448772 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
