Studies of cytochrome b-245 translocation in the PMA stimulation of the human neutrophil NADPH-oxidase
- PMID: 2987348
Studies of cytochrome b-245 translocation in the PMA stimulation of the human neutrophil NADPH-oxidase
Abstract
The human neutrophil respiratory burst, activated by phorbol 12-myristate 13-acetate (PMA), results from specific receptor-ligand binding and activation of the NADPH-oxidase in the plasma membrane. The role of granule membrane constituents has been elucidated with neutrophils disrupted by nitrogen cavitation and then fractionated in Percoll gradients to resolve four postnuclear fractions: cytoplasm, light membranes or gamma fraction (site of the NADPH-oxidase), a light granule (beta) fraction containing putative constituents of the NADPH-oxidase (cytochrome b-245 and an associated flavoprotein), and a fraction of heavy granules. Cytochrome b-245 is localized to two pools of specific granules within the beta fraction as assessed by differing sedimentation in narrow Percoll gradients and translocates upon PMA-stimulation from one of these specific granule sub-pools to the plasma membrane where it exhibits no change in its midpoint redox potential. Translocation of cytochrome b-245 parallels O2-production initiated by PMA stimulation as assessed in the time course of each activity. The finding of increased amounts of the b cytochrome in cytoplast membranes relative to plasma membranes of unstimulated cells suggests that the cytoplasts, devoid of granules yet capable of O2-generation upon PMA-stimulation, are useful in assessing post-translocation events in the activation pathway of the NADPH-oxidase. These data support the hypothesis that translocation of NADPH-oxidase components from an intracellular granular pool contributes to respiratory burst expression.
Similar articles
-
Phorbol myristate acetate mediates redistribution of protein kinase C in human neutrophils: potential role in the activation of the respiratory burst enzyme.J Immunol. 1985 Sep;135(3):2057-62. J Immunol. 1985. PMID: 3160785
-
NADPH oxidase activity of neutrophil specific granules: requirements for cytosolic components and evidence of assembly during cell activation.Mol Genet Metab. 2004 Apr;81(4):313-21. doi: 10.1016/j.ymgme.2004.01.009. Mol Genet Metab. 2004. PMID: 15059619
-
Different subcellular localization of cytochrome b and the dormant NADPH-oxidase in neutrophils and macrophages: effect on the production of reactive oxygen species during phagocytosis.Cell Immunol. 1995 Mar;161(1):61-71. doi: 10.1006/cimm.1995.1009. Cell Immunol. 1995. PMID: 7867086
-
The respiratory burst of phagocytosis: biochemistry and subcellular localization.Immunol Lett. 1985;11(3-4):165-71. doi: 10.1016/0165-2478(85)90164-6. Immunol Lett. 1985. PMID: 3936781 Review.
-
Subcellular localization and dynamics of components of the respiratory burst oxidase.J Bioenerg Biomembr. 1988 Dec;20(6):637-51. doi: 10.1007/BF00762546. J Bioenerg Biomembr. 1988. PMID: 2854126 Review.
Cited by
-
Influence of type and opsonization of ingested particle on intracellular free calcium distribution and superoxide production by human neutrophils.Infect Immun. 1987 Aug;55(8):1784-91. doi: 10.1128/iai.55.8.1784-1791.1987. Infect Immun. 1987. PMID: 3038751 Free PMC article.
-
Identification of a gC1q-binding protein (gC1q-R) on the surface of human neutrophils. Subcellular localization and binding properties in comparison with the cC1q-R.J Clin Invest. 1995 Apr;95(4):1569-78. doi: 10.1172/JCI117830. J Clin Invest. 1995. PMID: 7706463 Free PMC article.
-
Molecular basis of activation and regulation of the phagocyte respiratory burst.Ann Rheum Dis. 1987 Apr;46(4):265-72. doi: 10.1136/ard.46.4.265. Ann Rheum Dis. 1987. PMID: 3036025 Free PMC article. Review.
-
Cytochrome b co-fractionates with gelatinase-containing granules in human neutrophils.Mol Cell Biochem. 1991 Jun 26;105(1):49-60. doi: 10.1007/BF00230374. Mol Cell Biochem. 1991. PMID: 1656202
-
Role of gelsolin in actin depolymerization of adherent human neutrophils.Mol Biol Cell. 1997 Jan;8(1):121-8. doi: 10.1091/mbc.8.1.121. Mol Biol Cell. 1997. PMID: 9017600 Free PMC article.