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. 2018 Jun 29;360(6396):1423-1429.
doi: 10.1126/science.aau0325. Epub 2018 May 24.

Structures of the fully assembled Saccharomyces cerevisiae spliceosome before activation

Rui Bai  1 , Ruixue Wan  1 , Chuangye Yan  1 , Jianlin Lei  1   2 , Yigong Shi  3   4
Affiliations

Structures of the fully assembled Saccharomyces cerevisiae spliceosome before activation

Rui Bai et al. Science. .

Abstract

The precatalytic spliceosome (B complex) is preceded by the pre-B complex. Here we report the cryo-electron microscopy structures of the Saccharomyces cerevisiae pre-B and B complexes at average resolutions of 3.3 to 4.6 and 3.9 angstroms, respectively. In the pre-B complex, the duplex between the 5' splice site (5'SS) and U1 small nuclear RNA (snRNA) is recognized by Yhc1, Luc7, and the Sm ring. In the B complex, U1 small nuclear ribonucleoprotein is dissociated, the 5'-exon-5'SS sequences are translocated near U6 snRNA, and three B-specific proteins may orient the precursor messenger RNA. In both complexes, U6 snRNA is anchored to loop I of U5 snRNA, and the duplex between the branch point sequence and U2 snRNA is recognized by the SF3b complex. Structural analysis reveals the mechanism of assembly and activation for the yeast spliceosome.

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