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. 1987 Nov;61(11):3641-4.
doi: 10.1128/JVI.61.11.3641-3644.1987.

Dissociation of NS5 from cell fractions containing West Nile virus-specific polymerase activity

J B Grun  1 , M A Brinton
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Dissociation of NS5 from cell fractions containing West Nile virus-specific polymerase activity

J B Grun et al. J Virol. 1987 Nov.

Abstract

West Nile virus replication complexes were partially purified from cytoplasmic extracts of virus-infected cells by centrifugation through a 20% glycerol cushion. Numerous cell proteins, as well as the largest nonstructural protein, NS5, were separated from the replication complexes without significant loss of in vitro West Nile virus polymerase activity.

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