Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986;49(3):311-21.
doi: 10.1016/0378-1119(86)90367-7.

Dual-origin plasmids containing an amplifiable ColE1 ori; temperature-controlled expression of cloned genes

E M Wright, G O Humphreys, G T Yarranton

Dual-origin plasmids containing an amplifiable ColE1 ori; temperature-controlled expression of cloned genes

E M Wright et al. Gene. 1986.

Abstract

Dual-origin plasmids comprising an inducible ColE1-derived origin of replication controlled by the lambda pR promoter, the cI857 temperature-sensitive repressor gene and the pSC101 origin of replication and its associated par sequence, were constructed. Such plasmids carrying cloned genes were stably maintained at four copies per chromosome, and were readily amplifiable by thermal induction. Cloned gene expression increased with copy number, and accumulation values of greater than 20% total cellular protein were detected. These vectors should prove useful for the production of foreign protein on a large scale, since they provide for stable plasmid maintenance during the growth phase, and high-level gene expression without plasmid loss during the production phase.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

LinkOut - more resources