Review
doi: 10.1111/j.1768-322x.1986.tb00458.x.
Mitochondrial analysis in living cells: the use of rhodamine 123 and flow cytometry
- PMID: 2945606
- DOI: 10.1111/j.1768-322x.1986.tb00458.x
Item in Clipboard
Review
Mitochondrial analysis in living cells: the use of rhodamine 123 and flow cytometry
Biol Cell.
1986.
Abstract
Flow cytometry combines the advantages of microscopy and biochemical analysis in a single highly sensitive technique for a rapid examination of numerous individual living cells. It has become a potent and essential tool in the studies of the physiology of the whole cell and its organelles. Rhodamine 123 is a vital fluorescent dye used in flow cytometry. As it is specifically concentrated in mitochondria because of the transmembrane potential that these organelles maintain in living cells, rhodamine 123 is thus a useful probe for monitoring the abundance and activity of mitochondria. A critical survey of the routine use of rhodamine 123 together with flow cytometry in mitochondrial research is presented.
Similar articles
-
Increased uptake and retention of rhodamine 123 by mitochondria of old human fibroblasts.Mech Ageing Dev. 1987 Jun;39(1):1-9. doi: 10.1016/0047-6374(87)90081-9. Mech Ageing Dev. 1987. PMID: 3613685
-
Mitochondrial uptake of rhodamine 123 by rabbit articular chondrocytes.Cytometry. 1986 May;7(3):281-5. doi: 10.1002/cyto.990070309. Cytometry. 1986. PMID: 3709308
-
Unusual retention of rhodamine 123 by mitochondria in muscle and carcinoma cells.Proc Natl Acad Sci U S A. 1982 Sep;79(17):5292-6. doi: 10.1073/pnas.79.17.5292. Proc Natl Acad Sci U S A. 1982. PMID: 6752944 Free PMC article.
-
Cell membrane potential analysis.Methods Cell Biol. 1994;41:121-33. doi: 10.1016/s0091-679x(08)61713-6. Methods Cell Biol. 1994. PMID: 7532258 Review. No abstract available.
-
Fluorescent labeling of mitochondria.Methods Cell Biol. 1989;29:103-23. doi: 10.1016/s0091-679x(08)60190-9. Methods Cell Biol. 1989. PMID: 2643756 Review. No abstract available.
Cited by
-
Flow cytometric assessment of cell viability: a multifaceted analysis.Cytotechnology. 1989 Feb;2(1):27-37. doi: 10.1007/BF00365412. Cytotechnology. 1989. PMID: 22358578
-
Modulation of mitochondrial electrical potential: a candidate mechanism for drug resistance in African trypanosomes.Biochem J. 1997 Sep 15;326 ( Pt 3)(Pt 3):755-61. doi: 10.1042/bj3260755. Biochem J. 1997. PMID: 9307025 Free PMC article.
-
Cytofluorometric analysis of chondrotoxicity of fluoroquinolone antimicrobial agents.Antimicrob Agents Chemother. 1994 Feb;38(2):243-7. doi: 10.1128/AAC.38.2.243. Antimicrob Agents Chemother. 1994. PMID: 8192451 Free PMC article.
-
Isolated Mitochondria State after Myocardial Ischemia-Reperfusion Injury and Cardioprotection: Analysis by Flow Cytometry.Life (Basel). 2023 Mar 6;13(3):707. doi: 10.3390/life13030707. Life (Basel). 2023. PMID: 36983862 Free PMC article.
-
Eugenol Induces Phenotypic Alterations and Increases the Oxidative Burst in Cryptococcus.Front Microbiol. 2017 Dec 7;8:2419. doi: 10.3389/fmicb.2017.02419. eCollection 2017. Front Microbiol. 2017. PMID: 29270159 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
