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. 1986 Aug 29;139(1):64-70.
doi: 10.1016/s0006-291x(86)80080-8.

ADP-ribosylation in cultured cells treated with Clostridium difficile toxin B

I Florin, M Thelestam

ADP-ribosylation in cultured cells treated with Clostridium difficile toxin B

I Florin et al. Biochem Biophys Res Commun. .

Abstract

In cultured fibroblasts intoxicated with Clostridium difficile toxin B, a radioactive moiety was transferred from [14C-adenosine]NAD, but not from [14C-nicotinamide] NAD, into a cellular protein (MW 90,000). No labeling was detected in toxin-treated cultures not yet showing any toxin-induced cytopathogenic effect, whereas maximal labeling was obtained in cultures with about half of the cells showing a cytopathogenic effect. The radioactivity was removed from the substrate by treatment with snake venom phosphodiesterase. The results suggest that ADP-ribosylation of a cellular protein occurs in toxin B-treated cells and that this reaction may be responsible for development of the cytopathogenic effect.

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