Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1986 Feb;83(4):1060-4.
doi: 10.1073/pnas.83.4.1060.

Similarity between the interleukin 1 receptors on a murine T-lymphoma cell line and on a murine fibroblast cell line

S K Dower, S M Call, S Gillis, D L Urdal
Comparative Study

Similarity between the interleukin 1 receptors on a murine T-lymphoma cell line and on a murine fibroblast cell line

S K Dower et al. Proc Natl Acad Sci U S A. 1986 Feb.

Abstract

Interleukin 1 beta (IL-1 beta), one of two different polypeptide hormones with interleukin 1 (IL-1) biological activity, produced by activated human monocytes, is a 17.5-kDa protein. IL-1 beta binds specifically to a variety of cells; the cellular distribution of binding is consistent with reported biological responsiveness. In this report we show that two unrelated, but IL-1-responsive, cell lines, LBRM-33-1A5, a T-lymphoma line, and BALB/3T3, a fibroblast line, bind 125I-labeled IL-1 beta via similar plasma membrane receptor molecules. The T-lymphoma cells possess 238 +/- 16 plasma membrane receptors per cell and bind 125I-labeled IL-1 beta with an affinity of 3.6 +/- 0.9 X 10(9) M-1. The IL-1 receptor has a molecular size of approximately equal to 79.5 kDa, as estimated by affinity cross-linking. The fibroblasts possess 4.8 +/- 0.5 X 10(3) IL-1 receptors per cell and bind 125I-labeled IL-1 beta with an affinity of 2.6 +/- 0.5 X 10(9) M-1. The molecular size of the receptor molecule on the fibroblasts is approximately equal to 78 kDa. Despite the similarity in the characteristics of the ligand-receptor system on the two different cell types, the biological responses of the two cell types to IL-1 beta occur at IL-1 beta concentrations that differ by four orders of magnitude.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Adv Protein Chem. 1976;30:251-451 - PubMed
    1. Nature. 1956 Sep 29;178(4535):697-8 - PubMed
    1. J Immunol. 1978 Jun;120(6):2027-32 - PubMed
    1. Curr Top Cell Regul. 1978;13:259-311 - PubMed
    1. J Cell Biol. 1979 Dec;83(3):562-75 - PubMed

Publication types