Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Sep;76(9):4345-9.
doi: 10.1073/pnas.76.9.4345.

Eukaryotic mRNA cap binding protein: purification by affinity chromatography on sepharose-coupled m7GDP

N Sonenberg, K M Rupprecht, S M Hecht, A J Shatkin

Eukaryotic mRNA cap binding protein: purification by affinity chromatography on sepharose-coupled m7GDP

N Sonenberg et al. Proc Natl Acad Sci U S A. 1979 Sep.

Abstract

A 24,000-dalton polypeptide that binds strongly and can be specifically crosslinked to the 5'-terminal cap structure m7GpppN in eukaryotic mRNAs has been detected in protein synthesis initiation factor preparations [Proc. Natl. Acad. Sci. USA (1978) 75, 4843--4847]. This polypeptide has been purified to apparent homogeneity by one chromatographic passage through an affinity resin prepared by coupling the levulinic acid O2',3'-acetal of m7GDP to AH-Sepharose 4B. Translation, in HeLa cell extracts, of capped mRNAs including Sindbis virus, reovirus, and rabbit globin mRNAs was stimulated by the cap-binding protein under conditions that did not increase translation of noncapped RNAs of encephalomyocarditis virus and satellite tobacco necrosis virus.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1978 Feb;75(2):650-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2734-8 - PubMed
    1. FEBS Lett. 1978 Apr 1;88(1):21-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3040-4 - PubMed
    1. J Biol Chem. 1977 Apr 25;252(8):2684-90 - PubMed

LinkOut - more resources