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. 2017 Dec;591(23):3831-3841.
doi: 10.1002/1873-3468.12903. Epub 2017 Nov 22.

Mitochondrial helicase Irc3 translocates along double-stranded DNA

Tiina Sedman  1 , Natalja Garber  1 , Ilja Gaidutšik  1 , Sirelin Sillamaa  1 , Joosep Paats  1 , Vlad J Piljukov  1 , Juhan Sedman  1
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Free article

Mitochondrial helicase Irc3 translocates along double-stranded DNA

Tiina Sedman et al. FEBS Lett. 2017 Dec.
Free article

Abstract

Irc3 is a superfamily II helicase required for mitochondrial DNA stability in Saccharomyces cerevisiae. Irc3 remodels branched DNA structures, including substrates without extensive single-stranded regions. Therefore, it is unlikely that Irc3 uses the conventional single-stranded DNA translocase mechanism utilized by most helicases. Here, we demonstrate that Irc3 disrupts partially triple-stranded DNA structures in an ATP-dependent manner. Our kinetic experiments indicate that the rate of ATP hydrolysis by Irc3 is dependent on the length of the double-stranded DNA cosubstrate. Furthermore, the previously uncharacterized C-terminal region of Irc3 is essential for these two characteristic features and forms a high affinity complex with branched DNA. Together, our experiments demonstrate that Irc3 has double-stranded DNA translocase activity.

Keywords: DNA helicase; mitochondria; yeast.

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