Minocycline Directly Enhances the Self-Renewal of Adult Neural Precursor Cells
- PMID: 29081002
- DOI: 10.1007/s11064-017-2422-6
Minocycline Directly Enhances the Self-Renewal of Adult Neural Precursor Cells
Abstract
Minocycline not only has antibacterial action but also produces a variety of pharmacological effects. It has drawn considerable attention as a therapeutic agent for symptoms caused by inflammation in many neurological disorders, leading to several clinical trials. Although some of these effects are mediated through its function of suppressing microglial activation, it is not clear whether minocycline acts on other cell types in the adult brain. In this study, we utilized a colony-forming neurosphere assay, in which neural stem cells (NSCs) clonally proliferate to form floating colonies, called neurospheres. We found that minocycline (at therapeutically relevant concentrations in cerebrospinal fluid) enhances the self-renewal capability of NSCs derived from the subependymal zone of adult mouse brain and facilitates their differentiation into oligodendrocytes. Importantly, these effects were independent of a suppression of microglial activation and were specifically observed with minocycline (among tetracycline derivatives). In addition, the size of the NSC population in the adult brain was increased when minocycline was infused into the lateral ventricle by an osmotic minipump in vivo. While precise molecular mechanisms of how minocycline alters the behavior of adult NSCs remain unknown, our data provide a basis for the clinical use of minocycline to treat neurodegenerative and demyelinating diseases.
Keywords: Microglia; Neural stem cell; Oligodendrocyte; Self-renewal.
Similar articles
-
Effects of minocycline on endogenous neural stem cells after experimental stroke.Neuroscience. 2012 Jul 26;215:174-83. doi: 10.1016/j.neuroscience.2012.04.036. Epub 2012 Apr 24. Neuroscience. 2012. PMID: 22542871
-
Minocycline mitigates the gliogenic effects of proinflammatory cytokines on neural stem cells.J Neurosci Res. 2016 Feb;94(2):149-60. doi: 10.1002/jnr.23686. Epub 2015 Nov 3. J Neurosci Res. 2016. PMID: 26525774
-
The Neurofilament-Derived Peptide NFL-TBS.40-63 Targets Neural Stem Cells and Affects Their Properties.Stem Cells Transl Med. 2016 Jul;5(7):901-13. doi: 10.5966/sctm.2015-0221. Epub 2016 May 13. Stem Cells Transl Med. 2016. PMID: 27177578 Free PMC article.
-
Temporal and partial inhibition of GLI1 in neural stem cells (NSCs) results in the early maturation of NSC derived oligodendrocytes in vitro.Stem Cell Res Ther. 2019 Aug 27;10(1):272. doi: 10.1186/s13287-019-1374-y. Stem Cell Res Ther. 2019. PMID: 31455382 Free PMC article.
-
Pretreatment of aripiprazole and minocycline, but not haloperidol, suppresses oligodendrocyte damage from interferon-γ-stimulated microglia in co-culture model.Schizophr Res. 2013 Dec;151(1-3):20-8. doi: 10.1016/j.schres.2013.09.011. Epub 2013 Oct 4. Schizophr Res. 2013. PMID: 24100191
Cited by
-
The therapeutic role of minocycline in Parkinson's disease.Drugs Context. 2019 Mar 6;8:212553. doi: 10.7573/dic.212553. eCollection 2019. Drugs Context. 2019. PMID: 30873213 Free PMC article. Review.
References
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources