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Review
. 2017 Jul-Aug;51(4):582-594.
doi: 10.7868/S0026898417040036.

[CRISPR/CAS9, the King of Genome Editing Tools]

[Article in Russian]
A V Bannikov  1   2 , A V Lavrov  1   3
Affiliations
Free article
Review

[CRISPR/CAS9, the King of Genome Editing Tools]

[Article in Russian]
A V Bannikov et al. Mol Biol (Mosk). 2017 Jul-Aug.
Free article

Abstract

The discovery of CRISPR/Cas9 brought a hope for having an efficient, reliable, and readily available tool for genome editing. CRISPR/Cas9 is certainly easy to use, while its efficiency and reliability remain the focus of studies. The review describes the general principles of the organization and function of Cas nucleases and a number of important issues to be considered while planning genome editing experiments with CRISPR/Cas9. The issues include evaluation of the efficiency and specificity for Cas9, sgRNA selection, Cas9 variants designed artificially, and use of homologous recombination and nonhomologous end joining in DNA editing.

Keywords: CRISPR/Cas9; genome editing; sgRNA.

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