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. 2017 Oct;67(10):3872-3876.
doi: 10.1099/ijsem.0.002214. Epub 2017 Sep 8.

Borrelia lanei sp. nov. extends the diversity of Borrelia species in California

Gabriele Margos  1 , Natalia Fedorova  2 , Joyce E Kleinjan  2 , Christine Hartberger  1 , Tom G Schwan  3 , Andreas Sing  1 , Volker Fingerle  1
Affiliations

Borrelia lanei sp. nov. extends the diversity of Borrelia species in California

Gabriele Margos et al. Int J Syst Evol Microbiol. 2017 Oct.

Abstract

The diversity of Borrelia species discovered in California appears to be particularly high. A divergent group of Borrelia strains collected from Ixodes ticks in California was described by Postic and co-workers and designated 'genomospecies 2' (Postic D, Garnier M, Baranton G. Int J Med Microbiol 2007;297:263-271; Postic D, Ras NM, Lane RS, Hendson M, Baranton G. J Clin Microbiol 1998;36:3497-3504). We performed multilocus sequence analysis (MLSA) using eight housekeeping loci (clpA, clpX, nifS, pepX, pyrG, recG, rplB and uvrA) on 12 strains of this Borreliagenospecies to confirm that these strains form a distinct group within the Borreliaburgdorferi s. l. complex (Margos G, Hojgaard A, Lane RS, Cornet M, Fingerle V et al.Ticks Tick Borne Dis 2010;1:151-158). Phylogenetic and genetic distance analyses based on sequences of the MLSA housekeeping genes corroborated the distinctness of this group; genetic distances to all other members of the B. burgdorferi s.l. complex were 96 % or lower. We propose the name Borrelia lanei sp. nov. for this genospecies in honor of Professor Robert S. Lane, University of California Berkeley, for his contributions to Borrelia and tick research. The type strain for Borrelia lanei sp. nov., strain CA28-91T, has been deposited to two culture collections (=DSM 17992T=CIP 109135T).

Keywords: Borrelia burgdorferi sensu lato; MLSA; species complex; tick-borne bacteria.

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Conflict of interest statement

The authors declare that there are no conflicts of interest.

Figures

Fig. 1.
Fig. 1.
Molecular phylogenetic analysis of B. lanei sp. nov. The evolutionary history was inferred by using the maximum-likelihood method based on the general time reversible model [10]. The tree with the highest log likelihood (−33644, 4319) is shown. A discrete Gamma distribution was used to model evolutionary rate differences among sites [4 categories (+G, parameter=0, 6690)]. The rate variation model allowed for some sites to be evolutionarily invariable [(+I), 32, 8618 % sites]. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site (scale bar). B. lanei sp. nov. strains form a cluster containing two sister clades. Both sister clades form a separate cluster distinct from all other Borrelia genospecies known to date underlining this species is divergent.
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