Procedures and applications of long-term intravital microscopy
- PMID: 28669866
- DOI: 10.1016/j.ymeth.2017.06.029
Procedures and applications of long-term intravital microscopy
Abstract
Intravital microscopy (IVM) is increasingly used in biomedical research to study dynamic processes at cellular and subcellular resolution in their natural environment. Long-term IVM especially can be applied to visualize migration and proliferation over days to months within the same animal without recurrent surgeries. Skin can be repetitively imaged without surgery. To intermittently visualize cells in other organs, such as liver, mammary gland and brain, different imaging windows including the abdominal imaging window (AIW), dermal imaging window (DIW) and cranial imaging window (CIW) have been developed. In this review, we describe the procedure of window implantation and pros and cons of each technique as well as methods to retrace a position of interest over time. In addition, different fluorescent biosensors to facilitate the tracking of cells for different purposes, such as monitoring cell migration and proliferation, are discussed. Finally, we consider new techniques and possibilities of how long-term IVM can be even further improved in the future.
Keywords: Abdominal imaging window; Cranial imaging window; Dermal imaging window; Dorsal skinfold chamber; Long-term intravital microscopy; Skin.
Copyright © 2017 Elsevier Inc. All rights reserved.
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